Determination of the Homocysteine, Cysteine and Methionine in Human Plasma by Gas Chromatography with Electron Capture Detector

Myung, Seung-Woon; 명승운; Chang, Yoon-Jung; 장윤정; Yoo, Eun-Ah; 유은아; Park, Joon-Ho; 박준호; Min, Hye-Ki; 민혜기; Kim, Myung-Soo; 김명수

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P-ISSN1225-0163
E-ISSN2288-8985
SCOPUS, ESCI, KCI

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P-ISSN 1225-0163
E-ISSN 2288-8985

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12권 5호

PubReader Citation

기체크로마토그래피-전자포획검출기를 이용한 혈장 중의 Homocysteine, Cysteine 및 Methionine의 동시 분석법

Determination of the Homocysteine, Cysteine and Methionine in Human Plasma by Gas Chromatography with Electron Capture Detector

분석과학 / Analytical Science and Technology, (P)1225-0163; (E)2288-8985

1999, v.12 no.5, pp.408-414

명승운
장윤정
유은아
박준호
민혜기
김명수

명승운, 장윤정, 유은아, 박준호, 민혜기, & 김명수. (1999). 기체크로마토그래피-전자포획검출기를 이용한 혈장 중의 Homocysteine, Cysteine 및 Methionine의 동시 분석법, 12(5), 408-414.

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초록

기체 크로마토그래피-전자포획검출기 (gas chromatograph-electron capture detector : GC-ECD)를 이용하여 사람의 혈액 내에 존재하는 homocysteine (Hcy), 관련 화합물인 cysteine (Cys)과 methionine (Met)에 대해 선택적이고 감도 좋은 분석방법에 대하여 연구하였다. 시료는 혈장 내에서 환원과정을 거친 후 ethyl chloroformate (ECF)와 pentafluoropropyl alcohol (PEP-OH) 유도체화 시약을 사용하여 N(O,S)-ethylearhonyl pentafluoropropyl (PEP) ester 유도체를 만들어 추출의 효율을 높이면서 동시에 GC-ECD에 대한 크로마토그래피 성질을 향상시켰다. 생성된 유도체들은 chloroform으로 추출한 후 잔류물을 완전히 건조시키고 hexane으로 추출하여 GC-ECD로 분석하였다. Hcy와 Met는 $5-50{\mu}mol/L$, Cys는 $40-400{\mu}mol/L$ 범위에서 $R^2

keywords: GC-EDD, homocysteine, cysteine, methionine

Abstract

A selective and sensitive method for the derivatization of total homocysteine (Hcy) and the related compounds in plasma by gas chromatograph (GC)-electron capture detector (ECD) has been developed. To determine total homocysteine, cysteine (Cys), and methionine (Met) in human plasma using GC-ECD, analytes were reduced and converted into their N(O,S)-ethylearbonyl pentafluoropropyl (PFP) ester by derivatization with ethyl chloroformate and pentafluoropropyl alcohol (PFP-OH) in plasma. The best derivatizing agent N(O,S)-ethyl carbonyl PFP ester, was chosen by comparing the sensitivity of derivatized analysis in GC-ECD. The derivatized analytes in plasma were extracted by chloroform, and subsequently back-extracted with hexane and analyzed by GC-ECD. The calibration carves ($R^2$ > 0.990) were linear over the range $5-50{\mu}mol/L$ of Hcy and Met, $40-400{\mu}mol/L$ of Cys spiked in plasma. The detection limit observed by the established method was below $0.5{\mu}mol/L$. This method is highly sensitive and specific in the analysis of Hcy, Cys, and Met. Therefore, we suggest that this method is appropriate in the analysis of trace concentration of Hcy, Cys, and Met in biological fluids.

keywords: GC-EDD, homocysteine, cysteine, methionine

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기체크로마토그래피-전자포획검출기를 이용한 혈장 중의 Homocysteine, Cysteine 및 Methionine의 동시 분석법

Determination of the Homocysteine, Cysteine and Methionine in Human Plasma by Gas Chromatography with Electron Capture Detector

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