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  • P-ISSN1225-0163
  • E-ISSN2288-8985
  • SCOPUS, ESCI, KCI

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  • P-ISSN 1225-0163
  • E-ISSN 2288-8985

질량분석기를 단백질 분석에 적용하기 위한 고성능액체크로마토그래피 최적조건 연구

A Study on the potimal HPLC condition for peptides complex analysis using mass spectrometry

분석과학 / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2003, v.16 no.1, pp.78-81
박철홍 (바임 래버러토리즈)
권성원 (텍사스 주립대 사우스웨스턴 메디컬 센터)

Abstract

Peptides separation in high performance liquid chromatography (HPLC) is very important for the analysis of total proteins using mass spectrometry rather than two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). In this study, we investigated the optimal HPLC condition of peptides for the use of mass fingerprinting. As a result of pursuing a combination of solvent additives for HPLC, water and acetonitile containing both 0.1% trifluoroacetic acid and 0.1% acetic acid respectively showed the most efficient resolution and sensitivity.

keywords
liquid chromatography, mass spectrometry, peptides, proteomics

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