- P-ISSN 1225-0163
- E-ISSN 2288-8985
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
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Synthesis of Molecularly Imprinted Polymer (MIP) by Radiation-Induced Polymerization and Separation of Ferulic Acid from Rice Oil Using Mip-packed
분석과학 / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2006, v.19 no.3, pp.218-225
(Hannam University)
(한남대학교)
(한남대학교)
(한남대학교)
(한국생명공학연구원)
(한국생명공학연구원)
(경북대학교)
(한남대학교)
(한남대학교)
(한남대학교)
(한국생명공학연구원)
(한국생명공학연구원)
(경북대학교)
Seok-Kee,
Y.
, Jae-Chan,
L.
, 이승호,
최성호,
Hwa-Jung,
K.
, Hae-Jun,
P.
, &
강희동.
(2006). . , 19(3), 218-225.
Abstract
A molecularly imprinted polymer (MIP) was synthesized by radiation-induced polymerization (RIP), where the ferulic acid was used as a template molecule, 4-vinylpyridine as a monomer and ethylene glycoldimethacrylate (EGDMA) as a cross-linking monomer. The MIP was packed in a glass column using a slurry method for use in medium pressure liquid chromatography (MPLC). The MPLC column was tested for separation and purification of ferulic acid from the rice oil. When repeated three times, the MPLC separation/purification yielded the ferulic acid with the purity higher than ~99%. The chemiluminescence of the luminal (5-amino-2,3- dihydro-1,4-phtalazinedione) measured on a potato disc slide (5.0 mm thick) was enhanced in the presence of ferulic acid, while, without the ferulic acid, the chemiluminescence of luminol on the potato slice disc was not observed, which suggests the ferulic acid obtained from the rice oil can be useful for immunoassay.
- keywords
- Molecularly imprinted polymer (MIP), Radiation-induced polymerization (RIP), Ferulic acid, Rice oil, Chemiluminescence, Potato
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