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  • P-ISSN1225-0163
  • E-ISSN2288-8985
  • SCOPUS, ESCI, KCI

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  • P-ISSN 1225-0163
  • E-ISSN 2288-8985

논문 상세

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    Extraction and separation of glabridin from licorice by reversed phase high performance liquid chromatography

    분석과학 / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
    2006, v.19 no.6, pp.455-459
    노경호 (인하대학교)
    최두영 (육군3사관학교)
    노경호, & 최두영. (2006). . , 19(6), 455-459.

    Abstract

    The extraction and separation of glabridin from licorice root by HPLC was performed in this work. First, by investigating the different extraction solvents, extraction methods and extraction times, a one-hour ultrasonic extraction procedure with ethyl acetate as the extraction solvent was optimized. Then the ethyl acetate extraction was applied to RP-HPLC for separation of glabridin. The column efficiencies and resolutions were experimentally investigated with different mobile phase compositions. Baseline separation of glabridin was obtained under the mobile phase composition of 50/50 vol.% (ACN/water). The retention time of glabridin was 20.3 min. The peak of glabridin was collected from the HPLC elution for several times and identified by LC/MS. Under the optimum extraction and HPLC separation methods, 1.26 g of glabridin per kg licorice root could be extracted.

    keywords
    glabridin, licorice, extraction, separation, mobile phase optimization

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