open access
메뉴
ISSN : 1225-3480
Random amplified polymorphic DNA (RAPD) marker and sequence analyses of the internal transcribed spacer (ITS) region of ribosomal DNA were used to assess phylogenetic relationships of four Korean oyster species. The average number of species-specific markers identified from five universal rice primers (URPs) by RAPD-PCR was 1.8 for Crassostrea gigas, 3.2 for C. nippona, 3.6 for C. ariakensis, and 4.6 for Ostrea denselamellosa. The length of the ITS (ITS1-5.8S-ITS2) region ranged from 1,001 to 1,206 bp (ITS1, 426-518 bp; 5.8S, 157 bp; and ITS2, 418-536 bp), while the GC content ranged from 55.5-61.1% (ITS1, 56.8-61.8%; 5.8S, 56-57.3%; and ITS2, 54.1-62.2%). A phylogenetic analysis of the oysters based on our RAPD, ITS1, and ITS2 sequence data revealed a close relationship between C. gigas and C. nippona and a distant relationship between the genera Crassostrea and Ostrea. Our results indicated that RAPD and ITS sequence analysis was a useful tool for the elucidation of phylogenetic relationships and for the selection of species-specific markers in Korean oysters.
(1996). . Bulletin of Marine Science, 59, 196-208.
(1983). . Nucleic Acids Research, 11, 8111-8120.
(1985). . Evolution, 39, 783-791.
(1998). . Systematic Zoology, 37, 63-66.
(1996). . Fisheries Science, 62, 727-730.
(1992). . Molecular and General Genetics, 235, 157-165.
(2001). . Marine Biotechnology, 3, 355-361.
(2002). . Molecular and Cells, 13, 281-287.
(2000). . Marine Biotechnology, 2, 180-187.
(2004). . Aquaculture, 238, 1-37.
(1984). . Canadian Journal of Biochemistry and Cell Biology, 62, 311-320.
(1995). . Korean Journal of Systematic Zoology, 11, 235-242.
(2002). . Journal of Shellfish Research, 212, 697-705.
(1993). . Marine Ecology Progress Series, 96, 301-305.
(1997). . Journal of Korean Fisheries Society, 30, 804-808.
(2006). . Journal of Shellfish Research, 25, 727.
(2003). . Journal of Korean Fisheries Society, 36, 317-320.
(2000). . Marine Biotechnology, 2, 511-516.
(2001). . Journal of Food Science, 66, 657-661.
(2003). . Genome, 46, 595-604.
(2003). . Aquaculture, 228, 1-13.
(2006). . Journal of Shellfish Research, 25, 833-839.
(1999). . Molecular Ecology, 8, S65-S78.
(1998). . Bioinfomatics Application Notes, 14, 817-818.
(1999). . Bulletin of National Fisheries Research and Development Institute, 57, 73-78.
(2001). . Marine Biotechnology, 3, 133-144.
(2001). . Marine Biology, 139, 967-973.
(2006). . Journal of Shellfish Research, 25, 742.
(1998). . Journal of Molecular Evolution, 47, 406-419.
(1998). . Marine Ecology Progress Series, 162, 163-171.
(1997). . Molecular Biology and Evolution, 14, 465-473.
(2001). . Clinical and Diagnostic Laboratory Immunology, 8, 503-508.
(1998). .
(1990). . Nucleic Acids Research, 18, 6531-6535.
(2003). . Aquaculture, 228, 15-25.
(2004). . Aquaculture, 242, 137-155.
(1994). . Journal of Shellfish Research, 13, 547-553.
(2001). . Microbiology and Immunology, 45, 191-199.
(2000). . Korean Journal of Systematic Zoology, 16, 203-211.
(2005). . Marine Biotechnology, 7, 40-45.
(1997). . Molecular Marine Biology and Biotechnology, 6, 110-115.
(2004). . Briefings in Bioinformatics, 5, 150-163.
