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ISSN : 1225-3480

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Vol.25 No.2

Cellular Immune Responses of the Manila Clam, Ruditapes philippinarum, Exposed to Cadmium Chloride

Park, Kyung-Il
Choi, Min-Soon
Park, Sung-Woo
Park, Kwan-Ha
Choi, Sang-Hoon
Yoon, Jong-Man
The Korean Journal of Malacology / The Korean Journal of Malacology, (P)1225-3480;
2009, v.25 no.2, pp.135-143
Park,, K. , Choi,, M. , Park,, S. , Park,, K. , Choi,, S. , & Yoon,, J. (2009). Cellular Immune Responses of the Manila Clam, Ruditapes philippinarum, Exposed to Cadmium Chloride. , 25(2), 135-143.

Abstract

The Manila clam, Ruditapes philippinarum, has been considered as a sentinel species due to dominant distribution along the coast of Korea and well developed regulatory system. In order to develop and understand immune responses of the Manila clams, clams were exposed to $50\;{\mu}g/L$ of cadmium chloride (Cd) for 8 days and monitored the cellular immune parameters of the hemocytes including blast cell composition, DNA damage, necrosis, apoptosis and hemocyte mortality using a flow cytometer. The results showed that all immune parameters analyzed in the present study increased remarkably compared to the controls and the increases were statistically significant. Apoptosis rate was higher than necrosis rate in the clams exposed to Cd suggesting that apoptosis was preferably induced by the concentration of Cd used in the present study. Our study indicates that the measurement of cellular immune responses of the Manila clam using flow cytometer will be a useful technique for assessment of heavy metal contamination in marine environment.

keywords
ruditapes philippinarum, cadmium chloride, flow cytometry, immune parameter, environmental monitoring

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