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ISSN : 1225-3480

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26권 2호

피조개, Scapharca broughtonii (Schrenck) 정자의 냉장보존에 미치는 희석액의 효과

Effect of Diluents on the Cold Storage of Sperm in Scapharca broughtonii (Schrenck)

라성주 (전남대학교 수산해양대학 해양기술학부)
이성훈 (전남대학교 수산해양대학 해양기술학부)
고강희 (전남대학교 수산해양대학 해양기술학부)
한국패류학회지 / The Korean Journal of Malacology, (P)1225-3480;
2010, v.26 no.2, pp.145-149
라성주, 이성훈, & 고강희. (2010). 피조개, Scapharca broughtonii (Schrenck) 정자의 냉장보존에 미치는 희석액의 효과. , 26(2), 145-149.

초록

피조개, Scapharca broughtonii (Schrenck) 정자의 냉장보존을 위해 희석액에 따른 보존효과를 비교하였다. 피조개 정액을 채취하여 600 mM NaCl, Stein solution, 300 mM, 600 mM glucose (10 mM Hepes-pH 7.8), Ringer's solution (230 mM NaCl, 8 mM KCl, 2 mM $CaCl_2$, 3.7 mM $MgCl_2$, 0.2 mM $NaHCO_3$, 10 mM Hepes-pH 7.8), 20%, 25% ASW (NaCl 2.7 g + KCl 0.07 g + $CaCl_2$ 0.12 g + $MgCl_2$ 0.46 g + $NaHCO_3$ 0.05 g + distilled water 100 ml)를 각각 사용하여 $4^{\circ}C$에서 보관하였다. 피조개 정자의 보존효과는 정자의 운동성과 생존율로 평가였다. 피조개 정자는 Ringer's solution에서 7일간 냉장 보전하였을 때 운동성과 생존율을 가장 높았으며, 희석액의 적정 pH는 7.5 이었다.

keywords
Scapharca broughtonii, diluent, cold storage, sperm

Abstract

The effects of diluents composition on cold storage for Scapharca broughtonii (Schrenck) sperm were examined in the percentage of sperm activity and survival rate. Various diluents of glucose solutions (10 mM Hepes-pH 7.8), 600 mM NaCl, stein solution, Ringer's solution (230 mM NaCl, 8 mM KCl, 2 mM $CaCl_2$, 3.7 mM $MgCl_2$, 0.2 mM $NaHCO_3$, 10 mM Hepes-pH 7.8), 20%, 25% ASW (NaCl 2.7 g + KCl 0.07 g + $CaCl_2$ 0.12 g + $MgCl_2$ 0.46 g + $NaHCO_3$ 0.05 g + distilled water 100 ml) were used to store th sperm at $4^{\circ}C$. The storage effect was evaluated using sperm activity and survival rate. Ringer's solution was found to be better diluents which maintained high activity and survival rate of sperm for a storage period of 7 days. Optimal pH of diluents to store the sperm at $4^{\circ}C$ is 7.5.

keywords
Scapharca broughtonii, diluent, cold storage, sperm

참고문헌

1.

Adams, S.L.;Smith, J.F.;Roberts, R.D.;Janke, A.R.;Kaspar, H.F.;Robin Tervit, H.;Anne Pug. (2004). Cryopreservation of sperm of the Pacific oyster (Crassostrea gigas): development of a practical method for commercial spat production. Aquaculture, 242(1), 271-282. 10.1016/j.aquaculture.2004.08.034.

2.

Kawamoto, T.;Narita, T.;Isowa, K.;Aoki, H.;Hayashi, M.;Komaru, A.;Ohta, H.. (2007). Effects of cryopreservation methods on post-thaw motility of spermatozoa from the Japanese pearl oyster, Pinctada fucata martensii. Cryobiology, 54(1), 19-26. 10.1016/j.cryobiol.2006.10.190.

3.

(1997). . Bull. Nansei Natl. Fish. Res. Inst., 30, 115-123.

4.

Vuthiphandchai, V.;Thadsri, I.;Nimrat, S.. (2009). Chilled storage of walking catfish (Clarias macrocephalus) semen. Aquaculture, 296(1-2), 58-64. 10.1016/j.aquaculture.2009.07.018.

5.

Yao. (2000). . Aquaculture, 181(3-4), 361-375. 10.1016/S0044-8486(99)00240-9.

6.

(2006). . The Korean Journal of Malacology, 22(2), 175-182.

7.

(2007). . J. Aquaculture in Korea, 20(1), 31-40.

8.

McNiven, M.A.;Gallant, R.K.;Richardson, G.F.. (1993). Fresh storage of rainbow trout (Oncorhynchus mykiss) semen using a non-aqueous medium. Aquaculture, 109(1), 71-82. 10.1016/0044-8486(93)90487-J.

9.

MUCHLISIN. (2004). . Theriogenology, 62(1-2), 25-34. 10.1016/j.theriogenology.2003.05.006.

10.

Di Matteo, O.;Langellotti, A.L.;Masullo, P.;Sansone, G.. (2009). Cryopreservation of the Mediterranean mussel (Mytilus galloprovincialis) spermatozoa. Cryobiology, 58(2), 145-150. 10.1016/j.cryobiol.2008.09.015.

11.

Ohta. (1996). . Aquaculture, 142(1-2), 107-118. 10.1016/0044-8486(95)01246-X.

12.

(1998). . J. Kor. Fish. Soc., 31, 176-175.

13.

(2001). . J. Shellfish Research, 20, 51-59.

14.

(2003). . Dev. Reprod., 7(1), 9-13.

15.

Hauser, R.;Yogev, L.;Amit, A.;Yavetz, H.;Botchan, A.;Azem, F.;Lessing, J. B.;Ben-Yosef. (2005). Severe Hypospermatogenesis in Cases of Nonobstructive Azoospermia: Should We Use Fresh or Frozen Testicular Spermatozoa?. Journal of Andrology, 26(6), 772-778. 10.2164/jandrol.05044.

16.

He, S.;Woods III, L.C.. (2003). Effects of glycine and alanine on short-term storage and cryopreservation of striped bass (Morone saxatilis) spermatozoa. Cryobiology, 46(1), 17-25. 10.1016/S0011-2240(02)00159-1.

17.

Jing. (2009). . Aquaculture, 290(1-2), 165-171. 10.1016/j.aquaculture.2009.02.027.

18.

(2004). . Korean J. Ichthyol., 16(3), 215-222.

19.

Chao, N.-H.;Chen, H.-P.;Liao, I.-C.. (1975). Study on cryogenic preservation of grey mullet sperm. Aquaculture, 5(4), 389-406. 10.1016/0044-8486(75)90058-7.

20.

(1991). . Bull. Kor. Fish. Soc., 24, 70-78.

21.

DeGraaf. (2004). . Aquaculture, 234(1-4), 527-540. 10.1016/j.aquaculture.2003.11.037.

22.

Ding. (2009). . Animal Reproduction Science, 113(1-4), 229-235. 10.1016/j.anireprosci.2008.08.003.

23.

Donoghue. (2000). . Animal Reproduction Science, 62(1-3), 213-232. 10.1016/S0378-4320(00)00160-3.

24.

Fribourgh. (1966). . The Progressive Fish-Culturist, 28(4), 227-231. 10.1577/1548-8640(1966)28[227:TAOADS]2.0.CO;2.

25.

Friedler. (2002). . Human Reproduction, 17(12), 3114-3121. 10.1093/humrep/17.12.3114.

26.

HARA. (1982). . Aquaculture, 28(3-4), 339-346. 10.1016/0044-8486(82)90076-X.

27.

Alavi, S.M.H.;Cosson, J.. (2005). Sperm motility in fishes. I. Effects of temperature and pH: a review. Cell Biology International, 29(2), 101-110. 10.1016/j.cellbi.2004.11.021.

28.

(1953). . Nature, 172, 1189-1190.

29.

(1980). . Poultry Sci., 59, 428-437.

30.

Billard. (2004). . Aquaculture, 236(1-4), 1-9. 10.1016/j.aquaculture.2003.10.029.

31.

(1950). . Fertil. Steril., 1, 176-177.

32.

BUTTS. (2009). . Aquaculture, 286(1-2), 89-94. 10.1016/j.aquaculture.2008.09.005.

33.

Lanes, C.F.C.;Okamoto, M.;Cavalcanti, P.V.;Collares, T.;Campos, V.F.;Deschamps, J.C.;Rob. (2008). Cryopreservation of Brazilian flounder (Paralichthys orbignyanus) sperm. Aquaculture, 275(1-4), 361-365. 10.1016/j.aquaculture.2007.12.025.

34.

(2002). . J. Fish. Sci. Technol., 5, 64-74.

35.

(2005). . J. Kor. Fish. Soc., 38, 232-238.

36.

Zilli, L.;Schiavone, R.;Zonno, V.;Storelli, C.;Vilella, S.. (2003). Evaluation of DNA damage in Dicentrarchus labrax sperm following cryopreservation. Cryobiology, 47(3), 227-235. 10.1016/j.cryobiol.2003.10.002.

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