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굴, Crassostrea gigas 유생 성장단계별 미세조류 12의 소화도

Digestion indices of 12 species of microalgae by the oyster Crassostrea gigas larval development stages

초록

굴 인공종묘생산 시 적정 먹이생물을 선택하기 위하여 먹이생물로 이용되고 있는 12종의 미세조류를 대상으로 굴 유생의 소화도를 조사하였다. 조사는 유생의 발달단계에 따라 D형 유생, 초기각정기 유생, 각정기 유생 및 부착기 유생으로 구분하여 수용적 1L 수조에 5 마리/mL 밀도로 각각의 성장단계별 유생을 수용하고, 조사대상 먹이생물 12종을 각각 투여하였다. 소화도 측정은 먼저 12종의 먹이생물을 공급 후 3시간 동안 충분히 섭취하도록 방치한 후 먹이섭취 상태를 현광현미경으로 확인하였다. 이 후 유생은 걸름망으로 걸러 여과해수가 채워진 비이커에 재 수용 후 3,5 및 8시간 후 소화도를 현광현미경으로 측정하였다. 12종 미세조류의 소화도는 유생의 발달단계에 따라 다양했다. 전 유생기 동안 Thalassiosira weissflogii는 섭취가 관찰되지 않았고, 나머지 종의 소화도는 유생의 발달단계에 따라 0.8-99.7%: Chlorella ellipsoidea (0.8-5.4%), Nannochloris oculata (1.4-5.0%), Isochrysis galbana (99.1-99.5%), Pavlova lutheri (99.1-99.5%), I. aff. galbana (99.4-99.5%), Cheatoceros calcitrans (0.0-99.2%), C. gracilis (0.0-99.7%), C. simplex (0.0-95.9%), Phaeodactylum tricornutum (0.0-99.6%), Tetraselmis tetrathele (0.0-99.7%) 그리고 Dunaliella tertiolecta (0.0-99.6%)로 나타났다. 따라서 초기 유생은 I. galbana와 같이 소화성이 높은 것을 공급하고 각정기 이후 규조류 또는 담녹조류를 혼합해 주는 것이 소화성을 높일 수 있을 것으로 판단된다.

keywords
Digestion index, Epifluorescence microscopy, oyster, Crassostrea gigas, microalgae, larval development

Abstract

Twelve species of food microalgae were investigated to clarify the digestion index of Crassostrea gigas larvae using epifluorescence microscopy to choose an appropriate diet for artificial seed production in hatchery. An experiment was conducted using 1 (D shaped stage), 4 (Early umbo stage), 8 (umbo stage) and 12 (Full grown stage) days old larvae. larvae were stocked in 1 L flasks at 5 individuals/mL and fed $10{\times}10^4$ algal cells/mL of each species individually. Prior to larvae were fed for 3 h and then were observed under the microscope to detect ingestion; larvae were then sieved and replaced in 1 L flasks containing filtered seawater and were observed after 3, 5 and 8 h to analyse the digestion index. Values of digestion indices were specific for each alga. No evidence for the ingestion of Thalassiosira weissflogii was evident at all larval development stages tested. Digestion indices of others microalgae were 0.8-99.7% at 4 stage of larval development stages: Chlorella ellipsoidea (0.8-5.4%), Nannochloris oculata (1.4-5.0%), Isochrysis galbana (99.1-99.5%), Pavlova lutheri (99.1-99.5%), I. aff. galbana (99.4-99.5%), Cheatoceros calcitrans (0.0-99.2%), C. gracilis (0.0-99.7%), C. simplex (0.0-95.9%), Phaeodactylum tricornutum (0.0-99.6%), Tetraselmis tetrathele (0.0-99.7%) and Dunaliella tertiolecta (0.0-99.6%), respectively. Therefore, it is assumed that food microalgae showing the high digestion such as I. galbana should be supplied to the early umbo stage larvae, and then after the umbo larval stage, the mixed microalgae with diatoms and light green algae should be supplied to the full grown stage larvae to increase the digestion of their larvae.

keywords
Digestion index, Epifluorescence microscopy, oyster, Crassostrea gigas, microalgae, larval development

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