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ISSN : 1225-3480

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28권 1호

퍼킨서스편모충(Perkinsus olseni) 유주자 (Zoospore) 의 미세구조 관찰

Ultrastructure of Perkinsus olseni zoospores parasitizing the Manila clam Ruditapes philippinarum in Korea

김현중 (군산대학교 해양과학대학 수산생명의학과)
Gajamange, Dinesh (군산대학교 해양과학대학 수산생명의학과)
최민순 (군산대학교 해양과학대학 수산생명의학과)
최광식 (제주대학교 해양과학대학 해양의생명과학부)
박경일 (군산대학교 해양과학대학 수산생명의학과)
한국패류학회지 / The Korean Journal of Malacology, (P)1225-3480;
2012, v.28 no.1, pp.65-71
https://doi.org/10.9710/kjm.2012.28.1.065
김현중, Gajamange,, D. , 최민순, 최광식, & 박경일. (2012). 퍼킨서스편모충(Perkinsus olseni) 유주자 (Zoospore) 의 미세구조 관찰. , 28(1), 65-71, https://doi.org/10.9710/kjm.2012.28.1.065

초록

Perkinsus spp.는 국내를 비롯한 전 세계에 걸쳐 수산업적으로 중요한 이매패류에 감염되어 대량폐사를 유발하는 대표적 기생충이다. 본 연구에서는 바지락에서 검출되는 P. olseni의 유주자를 광학현미경과 주사전자현미경을 이용해 미세구조를 관찰한 결과 P. olseni의 유주자는 타원형의 몸체에 1개의 장편모와 1개의 단편모로 구성되어 있었으며, 장편모에는 섬모털을 보유하고 있었으며, 섬모털은 편모의 한쪽 면에만 분포하고 있었다. 유주자의 평균 체장은 $3.37{\pm}0.33{\mu}m$, 체폭은 $1.72{\pm}0.22{\mu}m$ 이었으며, 장편모는 $16.34{\pm}1.52{\mu}m$, 단편모는 $8.25{\pm}1.39{\mu}m$으로 측정되었다. 이는 유럽과 미국에서 보고된 Perkinsus spp.와 비교 하였을 때 몸체의 크기는 작지만 편모의 길이는 긴 것으로 확인되었다.

keywords
Perkinsus olseni, Manila clam, zoospore, flagella, mastigoneme, Korea

Abstract

Protozoan parasites belonging to the genus Perkinsus elicit severe inflammatory responses and are associated with mass mortality of commercially important marine shellfish worldwide. In the present study, we examined the external features of P. olseni zoospores in detail using light and scanning electron microscopy. Our study showed that the zoospores have an oval body with a long anterior flagellum and a short posterior flagellum. The anterior flagellum has a unilateral array of mastigonemes. Mean body dimensions were $3.37{\pm}0.33{\mu}m{\times}1.72{\pm}0.22{\mu}m$. The average length of the anterior and posterior flagella was $16.34{\pm}1.52{\mu}m$ and $8.25{\pm}1.39{\mu}m$, respectively. Zoospores of P. olseni found in Korean waters have shorter and narrower bodies, longer anterior flagella, and shorter posterior flagella than zoospores of Perkinsus spp. found in the mollusks of North America and Europe.

keywords
Perkinsus olseni, Manila clam, zoospore, flagella, mastigoneme, Korea

참고문헌

1.

(1996). Histology of Perkinsus marinus, a pathogen of oysters in Chesapeake Bay 1950-1984. Journal of Shellfish Research, 15(1), 13-16.

2.

(1995). In vitro sporulation of the clam pathogen Perkinsus atlanticus (Apicomplexa, Perkinsea) under various environmental conditions. Journal of Shellfish Research, 14, 469-475.

3.

(1989). Fine structure of Perkinsus atlanticus n. sp. (Apicomplexa, Perkinsea) parasite of the clam Ruditapes decussatus from Portugal. Journal of Parasitology, 75(4), 627-635. 10.2307/3282915.

4.

(1998). Perkinsus qugwadi sp. nov. (incertae sedis), a pathogenic protozoan parasite of Japanese scallops, Patinopecten yessoensis, cultured in British Columbia, Canada. Canadian Journal of Zoology, 76, 942-953. 10.1139/z98-015.

5.

Architecture and assembly of mastigonemes and related structures;Advances in Cell and Molecular Biology, Vol 2.

6.

(1996). Epizootiology of Perkinsus marinus disease of oyster in Chesapeake Bay, with emphasis on data since 1985. Journal of Shellfish Research, 15, 17-34.

7.

(2004). Perkinsus mediterraneus n. sp. a protistan parasite of the European flat oyster Ostrea edulis from the Balearic Islands, Mediterranean Sea. Diseases of Aquatic Organisms, 58, 231-244. 10.3354/dao058231.

8.

(1997). Report on occurrence of Perkinsus sp. in the Manila clam, Ruditapes philippinarum, in Korea. Journal of Aquaculture, 10, 227-237.

9.

(2005). Diagnosis, pathology, and taxonomy of Perkinsus sp. isolated from the Manila clam Ruditapes philippinarum in Korea. Journal of Aquaculture, 18, 207-214.

10.

(2001). Description of Perkinsus andrewsi n. sp. isolated from the Baltic clam (Macoma balthica) by characterization of the ribosomal RNA locus, and development of species-specific PCR-based diagnostic assay. Journal of Eukaryotic Microbiology, 48, 52-61. 10.1111/j.1550-7408.2001.tb00415.x.

11.

(2006). In vitro propagation of two Perkinsus spp. Parasites from Japanese Manila clam Venerupis philippinarum and description of Perkinsus honshuensis n. sp. Journal of Eukaryotic Microbiology, 53, 316-326. 10.1111/j.1550-7408.2006.00120.x.

12.

(1998). Perkinsus protozoan infection in short-necked clam Tapes (= Ruditapes) philippinarum in Japan. Fish pathology, 33, 473-480. 10.3147/jsfp.33.473.

13.

(2000). Zoosporulation of a new Perkinsus species isolated from the gills of the softshell clam Mya arenaria. Parasite, 7, 115-122. 10.1051/parasite/2000072115.

14.

(1981). A new Perkinsus species (Apicomplexa, Perkinsea) from the abalone, Haliotis ruber. Journal of Invertebrate Pathology, 37, 181-187. 10.1016/0022-2011(81)90073-2.

15.

(2001). Prevalence of Perkinsus sp. in the Manila clam, Ruditapes philippinarum, along the Northern coast of the Yellow Sea in China. Chinese Journal of Oceanologia et Limunologia Sinica, 32, 502-511.

16.

(1950). Preliminary note on occurrence of a new protistan parasite, Dermocystidium marinum n. sp., in Crassostrea virginica (Gmelin). Science, 111, 328-329. 10.1126/science.111.2883.328.

17.

(2008). Description of Perkinsus beihaiensis n. sp. a new Perkinsus sp. parasite in oysters of southern China. Journal of Eukaryotic Microbiology, 55, 117-130. 10.1111/j.1550-7408.2008.00314.x.

18.

(1998). In vitro culture of Perkinsus atlanticus, a parasite of the carpet shell clam Ruditapes decussatus. Diseases of Aquatic Organisms, 33, 129-136. 10.3354/dao033129.

19.

(2001). Spatial distribution of the protozoan parasite Perkinsus sp. found in the Manila clams, Ruditapes philippinarum, in Korea. Aquaculture, 203, 9-22. 10.1016/S0044-8486(01)00619-6.

20.

(2006). Application of enzyme-linked immunosorbent assay (ELISA) for the study of reproduction in the Manila clam, Ruditapes philippinarum: (Mollusca: Bivalvia): II. Impacts of Perkinsus olseni on clam reproduction. Aquaculture, 251, 182-191. 10.1016/j.aquaculture.2005.06.003.

21.

(2005). Use of molecular markers for species identification of Korean Perkinsus sp. isolated from Manila clam, Ruditapes philippinarum. Diseases of Aquatic Organisms, 66, 255-263. 10.3354/dao066255.

22.

(1968). Fine structure of zoospores from Labyrinthomyxa sp. parasitizing the clam Macoma balthica. Chesapeake Science, 9, 198-208. 10.2307/1351185.

23.

(1996). The structure of Perkinsus marinus (Mackin, Owen and Collier, 1950) Levine, 1978 with comments on taxonomy and phylogeny of Perkinsus sp. Journal of Shellfish Research, 15, 67-87.

24.

Studies on transmission and pathogenicity of Dermocystidium marinum. Texas A&M Research Foundation Project 23, Technical Report.

25.

(1967). Flagellar motion and fine structure of the flagella apparatus in Chlamydomonas. Journal of Cell Biology, 33, 543-571. 10.1083/jcb.33.3.543.

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