바로가기메뉴

본문 바로가기 주메뉴 바로가기
 
 

logo

메뉴
search

ISSN : 1225-3480

논문 상세

이전 다음
논문 투고

28권 4호

소라, Batillus cornutus (Lightfoot, 1786) 혈구의 종류 및 미세구조적 특징

Ultrastructural Characteristics and Categorization of the Hemocytes in Spiny Top shell, Batillus cornutus (Lightfoot, 1786) (Gastropoda: Turbinidae)

박정준 (국립수산과학원 양식관리과)
김석렬 (국립수산과학원 병리연구과)
이정식 (전남대학교 수산생명의학과)
박명애 (국립수산과학원 양식관리과)
김재원 (강원도립대학교 해양생명과학과)
정귀권 (영광정보산업고등학교)
한국패류학회지 / The Korean Journal of Malacology, (P)1225-3480;
2012, v.28 no.4, pp.321-328
https://doi.org/10.9710/kjm.2012.28.4.321
박정준, 김석렬, 이정식, 박명애, 김재원, & 정귀권. (2012). 소라, Batillus cornutus (Lightfoot, 1786) 혈구의 종류 및 미세구조적 특징. , 28(4), 321-328, https://doi.org/10.9710/kjm.2012.28.4.321

초록

전라남도 완도군 인근연안에서 채집된 소라, Batillus cornutus의 혈구를 광학현미경과 전자현미경을 이용하여 혈구의 종류 및 미세구조적인 특징을 연구하였다. 광학현미경 상에서 소라의 혈구들은 호염기성 세포로 관찰되었다. 혈구의 형태학적인 특징으로 분류를 하였을 때 소라의 혈구는 모두 8종류로 나눌 수 있었다. 미성숙 혈구는 직경 약 4.5-5.5 ${\mu}m$로서 세포질 내에 지름 2-3 ${\mu}m$인 원형의 핵이 대부분을 차지하고 있었다. 과립세포는 직경 약 7 ${\mu}m$로서 세포질에는 전자밀도가 낮고 크기가 다양한 과립들과 미토콘드리아, 글리코겐 과립들이 관찰되었다. 초자세포는 핵의 크기 및 세포질에 존재하는 미세소관의 특징에 따라 6종류로 세분화 할 수 있었다. 초자세포 III은 라이소좀 및 글리코겐 과립의 형성이 미약하여 식세포의 기능 보다는 영양분의 흡수 및 운반 기능을 수행하는 것으로 판단되며, 특히 초자세포 VI은 형태가 불규칙한 아메바형으로 세포질에 글리코겐 과립의 분포가 높고, 이식포와 자식포가 분포하고 있었다. 따라서 이들 혈구들의 정확한 기능을 파악하기 위해서는 효소학적 및 면역학적 연구를 추가적으로 수행해야 할 것으로 판단된다.

keywords
Batillus cornutus, hemocyte, granulocyte, hyalinocyte

Abstract

Light and transmission electron microscopy of Batillus cornutus hemocytes revealed differences that the morphological distinctions between blast-like cell, granulocytes and hyalinocytes. Base on the morphological characteristics of the cells, we identified the eight types of hemocytes and present a categorization of the hyalinocytes into six sub-categories. The hemocytes of B. cornutus were observed basophilic cell under the light microscopy. Blast-like cells had a spherical profile with a central nucleus filling almost the whole cell. Granulocytes were characterized by presenting variable numbers of granules. This cell had spherical shape with diameter 7 ${\mu}m$ and smooth endoplasmic reticula, granules, mitochondria, glycogen granules in the cytoplasm. Hyalinocytes were the most abundant cell type. Especially, hyalinocyte VI had iirregular an amoebal shape and observed autophagosome and heterophagosome in the cytoplasm. From these results, it is concluded that there are eight types of cells in the hemolymph of B. cornutus. Further studies are now needed to identify the role of these hemocytes in the enzymological and immunological response.

keywords
Batillus cornutus, hemocyte, granulocyte, hyalinocyte

참고문헌

1.

(1977). Hemocytes of the pulmonate gastropod Biomphalaria glabrata. Journal of Invertebrate Pathology, 30, 119-122. 10.1016/0022-2011(77)90052-0.

2.

(1970). Transplantation immunity in moluscs: the histoincompatibility of Helisoma duryi normale with allografts and xenografts. Journal of Invertebrate Pathology, 15, 177-192. 10.1016/0022-2011(70)90232-6.

3.

(1980). Behavior of Bulinus truncatus rohlfsi hemocytes (Gastropoda: Pulmonata). Transactions of the American Microscopical Society, 99, 101-111. 10.2307/3226085.

4.

(1972). Limpet haemocytes. I. Studies on aggregation and spike formation. Journal of Cell Science, 11, 757-769.

5.

(2010). First characterisation of the populations and immune-related activities of hemocytes from two edible gastropod species, the disk abalone, Haliotis discus discus and the spiny top shell, Turbo cornutus. Fish and Shellfish Immunology, 28, 87-97. 10.1016/j.fsi.2009.10.006.

6.

(1950). The blood of gastropod molluscs. Journla of Morphology, 86, 315-327. 10.1002/jmor.1050860205.

7.

(2000). Haliotis tuberculata hemocyanin (HtH): analysis of oligomoric stability of HtH1 and HtH2, and comparison with keyhole limpet hemocyanin KLH1 and KLH2. Micron, 31, 613-622. 10.1016/S0968-4328(99)00145-6.

8.

(1984). Cardiac output and tissue blood flow in the abalone, Haliotis cracherodii (Mollusca: Gastropoda). Journal of Experimental Zoology, 231, 309-324. 10.1002/jez.1402310303.

9.

(1999). Abalone (Haliotis tuberculata) hemocyanin type 1(HtH1). Organization of the <TEX>$\approx$</TEX>400 kDa subunit, and amino acid sequence of its functional units f, g and h. European Journal of Biochemistry, 264, 27-38. 10.1046/j.1432-1327.1999.00564.x.

10.

(2000). The sequence of a gastropod hemocyanin (HtH1 from Haliotis tuberculata). Journal of Biological Chemistry, 275, 5675-5681. 10.1074/jbc.275.8.5675.

11.

(1999). Subunit organization of the abalone Haliotis tuberculata hemocyanin type 2 (HtH2) and the cDNA sequence coding for its functional unit d, e, f, g and h. European Journal of Biochemistry, 265, 134-144. 10.1046/j.1432-1327.1999.00694.x.

12.

(2008). Categorization of hemocytes of three gastropod species Trachea vittata (Muller), Pila globosa (Swainson) and Indoplanoribis exustus (Dehays). Journal of Invertebrate Pathology, 97, 20-26. 10.1016/j.jip.2007.07.007.

13.

(1990). Cellular cadmium distribution in the common winkle, Littorina littorea (L.) determined by X-ray microprobe analysis and histochemistry. Histochemistry, 94, 191-199. 10.1007/BF02440187.

14.

(1999). Internal defenses of the snail Biomphalaria glabrata. Journal of Invertebrate Pathology, 74, 224-234. 10.1006/jipa.1999.4876.

15.

(2000). Structure of a molluscan hemocyanin didecamer (HtH1 from Haliotis tuberculata) at 12 A resolution by cryoelectron microscopy. Journal of Molecular Biology, 298, 21-34. 10.1006/jmbi.2000.3631.

16.

(1972). Host-parasite interrelationships between the freshwater pulmonate Biomphalaria pfeiferi and the trematode Schistosoma mansoni. Nethelands Journal of Zoology, 22, 355-427.

17.

(1988). Kidney and hemocytes of Mya arenaria (Bivalvia): normal and pollution-related ultrastructureal morphologies. Journal of Invertebrate Pathology, 52, 201-214. 10.1016/0022-2011(88)90127-9.

18.

(1989). Heamocytes of the freshwater snail Viviparus ater (Gastropoda: Prosobranchia). Journal of Mollusca. Studies, 55, 379-382. 10.1093/mollus/55.3.379.

19.

(1965). Variation of hemocyanin concentration in the blood of four species of Haliotis. Biological Bulletin, 128, 459-472. 10.2307/1539905.

20.

(1971). The fine structure of the granular amebocytes of the pacific oyster Crassostrea gigas. Journal of invertebrate Pathology, 18, 169-275. 10.1016/0022-2011(71)90144-3.

21.

Gastropods; Invertebrate Blood Cells.

22.

(1980). A comparative morphological and enzyme histochemical study on blood cell of the freshwater snails Lymnaea stagnalis, Biomphalaria glabrata, and Bulinus truncatus. Journal of Morphology, 165, 31-39. 10.1002/jmor.1051650104.

23.

Korean shells in color.

24.

(1976). The ultrastructure of circulating hemolymph cells of the marine snail Cerithidea californica (Gastropoda: Prosobranchiata). Journal of Morphology, 150, 485-494.

25.

(2012). Morphological characterization of hemocytes from Biomphalaria glabrata and Biomphalaria straminea. Mircon, 43, 285-291.

26.

(2001). Rhogocytes (pore cells) as the site of hemocyanin biosynthesis in the marine gastropod Haliotis tuberculata. Cell Tissue Research, 304, 455-462. 10.1007/s004410100368.

27.

(1993). Morphological characterization of the hemocytes of the pulmonate snail Biomphalaria tenagophila. Mememorias Do Institute Oswaldo Cruz, 88, 7.

28.

Molluscan immunobiology;The Mollusca Vol. 5, Physiology Part 2.

logo

논문 투고
OA 정책