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ISSN : 1225-3480

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31권 2호

β-glucan이 바지락의 면역력에 미치는 영향

Effect of β-glucan on immune parameters in the Manila clam Ruditapes philippinarum

남기웅 (군산대학교 해양과학대학 해양생명응용과학부 수산생명의학전공)
박경일 (군산대학교 해양과학대학 해양생명응용과학부 수산생명의학전공)
한국패류학회지 / The Korean Journal of Malacology, (P)1225-3480;
2015, v.31 no.2, pp.123-127
https://doi.org/10.9710/kjm.2015.31.2.123
남기웅, & 박경일. (2015). β-glucan이 바지락의 면역력에 미치는 영향. , 31(2), 123-127, https://doi.org/10.9710/kjm.2015.31.2.123

초록

${\beta}$-glucan은 면역증강제의 하나로 어류를 비롯한 척추동물의 사료첨가제에 널리 이용되고 있는 다당체이다. 본 연구는 척추동물과는 다른 면역체계를 갖고 있는 바지락의 면역반응에 ${\beta}$-glucan이 미치는 영향을 조사하기 위하여 실시되었다. 이를 위하여 식물플랑크톤이 공급된 해수에 0, 0.1, 1%의 ${\beta}$-glucan을 첨가하고 이 해수에 바지락을 매일 1시간 씩 2주간 노출시켜 먹이 섭식 시 ${\beta}$-glucan이 흡수되도록 하였다. 바지락의 면역력은 혈구의 식세포작용과 혈림프액의 병원성 세균에 대한 정균력을 실험전과 ${\beta}$-glucan을 급이한 2주 후 각 그룹별로 비교하였다. 실험결과 0.1%의 ${\beta}$-glucan에 노출된 바지락의 식세포율은 대조구와 비교하여 뚜렷한 증가가 관찰되지 않았으나 1%의 ${\beta}$-glucan에 노출된 경우 약 30%의 식세포율이 증가하였다. 또한 정균력에 관한 실험에서 ${\beta}$-glucan은 바지락 혈림프액이 Vibrio tapetis, V. parahaemolyticus, V. ordalii 등의 병원성 세균의 증식을 억제케 함이 확인되었다. 바지락의 사망률 역시 ${\beta}$-glucan에 노출된 바지락에서 낮았으며 이러한 경향은 ${\beta}$-glucan의 농도가 높을수록 낮았다. 본 연구를 통하여 바지락은 ${\beta}$-glucan에 의해 면역력이 상승하였으며, 주사방식이 아닌 해수 침지시에도 발생함을 확인하였다.

keywords
<tex> ${\beta}$</tex>-lucan, Ruditapes philippinarum, immune stimulation, antibacterial activity

Abstract

${\beta}$-Glucan is a polysaccharide that is widely used as an adductive in fish feed to facilitate immune stimulation. This study aimed to investigate the effect of ${\beta}$-glucan on immune responses in the Manila clam Ruditapes philippinarum. For this purpose, three groups of R. philippinarum were exposed to 0%, 0.1%, or 1% ${\beta}$-glucan in sea water for 1 hr/day for 2 weeks using an immersion method. Thereafter, two immune parameters-phagocytic rate and antibacterial activity-were measured. R. philippinarum exposed to 1% ${\beta}$-glucan showed an approximate 30% significant increase in phagocytic rate. In addition, ${\beta}$-glucan significantly limited the growth of the pathogenic bacteria Vibrio tapetis, V. parahaemolyticus, and V. ordalii. Moreover, the mortality rates of ${\beta}$-glucan-treated clams decreased during a 17-day experiment. Our study suggests that treatment with ${\beta}$-glucan significantly increases the immune responses in R. philippinarum, and that immersion is a simple and effective method for immune stimulation in this species.

keywords
<TEX>${\beta}$</TEX>-lucan, Ruditapes philippinarum, immune stimulation, antibacterial activity

참고문헌

1.

Ainsworth, A.J.. (1994). A <TEX>${\beta}$</TEX>-glucan inhibitable zymosan receptor on channel catfish neutrophils. Veterinary immunology and immunopathology, 41, 141-152. 10.1016/0165-2427(94)90063-9.

2.

Allam, B. and Raftos, D.. (0000). Immune responses to infectious diseases in bivalves. Journal of Invertebrate Pathology, .

3.

Aderem, A. and Ulevitch, R.J.. (2000). Toll-like receptors in the induction of the innate immune response. Nature, 406, 782-787. 10.1038/35021228.

4.

Brogden, G., Krimmling, T., Adame, K.M., Naim, H.Y., Steinhagen, D. and von Kockritz-Blickwede, M.. (2014). The effect of b-glucan on formation and functionality of neutrophil extracellular traps in carp (Cyprinus carpio L.). Developmental and Comparative Immunology, 44, 280-285. 10.1016/j.dci.2014.01.003.

5.

Costa, M.M., Novoa, B. and Figueras, A.. (2008). Influence of <TEX>${\beta}$</TEX>-glucans on the immune responses of carpet shell clam (Ruditapes decussatus) and Mediterranean mussel (Mytilus galloprovincialis). Fish & Shellfish Immunology, 24(5), 498-505. 10.1016/j.fsi.2007.10.003.

6.

Diao, J., Ye, H.B., Yu, X.Q., Xu, Y.F., La,. Li, T.B. and Wang Y.Q.. (2013). Adjuvant and immunostimulatory effects of LPS and <TEX>${\beta}$</TEX>-glucan on immune response in Japanese flounder, Paralichthys olivaceus. Veterinary Immunology and Immunopathology, 156, 167-175. 10.1016/j.vetimm.2013.10.004.

7.

Ghaedi, G., Keyvanshokooh, S., Azarm, H.M. and Akhlaghi, M.. (2015). Effects of dietary <TEX>${\beta}$</TEX>-glucan on maternal immunity and fry quality of rainbow trout (Oncorhynchus mykiss). Aquaculture, 441, 78-83. 10.1016/j.aquaculture.2015.02.023.

8.

Hetland, G., Johnson, E., Eide, D.M., Grinde, B., Samuelsen, A.B.C. and Wiker, H. G., ed. by Mendez-Vilas, A. Antimicrobial effects of <TEX>${\beta}$</TEX>-glucans and pectin and of the Agaricus blazei based mushroom extract, AndoSanTM. Examples of mouse models for pneumococcal-, fecal bacterial-, and mycobacterial infections. In: Microbial pathogens and strategies for combating them: science, technology and education Vol 2..

9.

Lopez-Joven, C., de Blas, I., Ruiz-Zarzuela, I., Furones, M.D. and Roque, A. (2011). Experimental uptake and retention of pathogenic and nonpathogenic Vibrio parahaemolyticus in two species of clams: Ruditapes decussatus and Ruditapes philippinarum. Journal of Applied Microbiology, 111(1), 197-208. 10.1111/j.1365-2672.2011.05024.x.

10.

Medzhitov, R. and Janeway, Jr. C.A.. (1997). Innate immunity: the virtues of a nonclonal system of recognition. Cell, 91, 295-298. 10.1016/S0092-8674(00)80412-2.

11.

Mueller, A., Raptis, J., Rice, P.J., Kalbfleisch, J.H., Stout, R.D., Ensley, H.E., Browder, W. and Williams, D.L. (2000). The influence of glucan polymer structure and solution conformation on binding to (1-->3)-beta-D-glucan receptors in a human monocyte-like cell line. Glycobiology, 10(4), 339-346. 10.1093/glycob/10.4.339.

12.

Park, K.I.. (2013). Variation of nitric oxide concentrations in response to shaking stress in the Manila clam Ruditapes philippinarum. Korean Journal of Malacology, 29, 1-6. 10.9710/kjm.2013.29.1.1.

13.

Ruiz, P., Poblete, M., Yanez, A.J., Irgang, R., Toranzo, A.E. and Avendano-Herrera, R. (2015). Cell-surface properties of Vibrio ordalii strains isolated from Atlantic salmon Salmo salar in Chilean farms. Diseases of Aquatic Organisms, 113, 9-23. 10.3354/dao02820.

14.

Park, K.-I., Paillard, C., Chevalier, P. and Choi, K.-S.. (2006). Report on the occurrence of brown ring disease (BRD) in Manila clam, Ruditapes philippinarum, on the west coast of Korea. Aquaculture, 255, 610-613. 10.1016/j.aquaculture.2005.12.011.

15.

Park, S.O. and Kim, J.. (2012). Functional food for immune regulation - beta-glucan. Food Science and Industry, 45, 39-47.

16.

Pipe, R.K.. (1992). Generation of reactive oxygen metabolites by the haemocytes of the mussel Mytilus edulis. Developmental and Comparative Immunology, 16, 111-122. 10.1016/0145-305X(92)90012-2.

17.

Sirimanapong, W., Adams, A., Ooi E.L., Green, M.D., Nguyen, D.K., Browdy, L,C., Collet, B. and Kim, D.T.. (2015). The effects of feeding immunostimulant <TEX>${\beta}$</TEX>-glucan on the immune response of Pangasianodon hypophthalmus. Fish & Shellfish Immunology, 45, 357-366. 10.1016/j.fsi.2015.04.025.

18.

Torreilles, J, Guerin, M.C. and Roch, P.. (1997). Peroxidase-release associated with phagocytosis in Mytilus galloprovincialis haemocytes. Developmental and Comparative Immunology, 21, 267-275. 10.1016/S0145-305X(96)00034-1.

19.

Tzianabos, A.O.. (2000). Polysaccharide immunomodulators as therapeutic agents:structural aspects and biologic function. Clinical Microbiology Reviews, 13, 523-533. 10.1128/CMR.13.4.523-533.2000.

20.

Wongsasak, U., Chaijamrus, S., Kumkhong, S. and Boonanuntanasarn, S.. (2015). Effects of dietary supplementation with <TEX>${\beta}$</TEX>-glucan and synbiotics on immune gene expression and immune parameters under ammonia stress in Pacific white shrimp. Aquaculture, 436, 179-187. 10.1016/j.aquaculture.2014.10.028.

21.

Zhao, Y., Ma, H., Zhang, W., Ai, Q., Mai, K., Xu, Wang, X. and Liufu, Z.. (2011). Effects of dietary <TEX>${\beta}$</TEX>-glucan on the growth, immune responses and resistance of sea cucumber, Apostichopus japonicus against Vibrio splendidus infection. Aquaculture, 315(3), 269-274. 10.1016/j.aquaculture.2011.02.032.

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