염주알다슬기 (Koreanomelania nodifila) 의 Arginine Kinase 유전자를 이용한 분자계통학적 분석
Hyeok Lee
(Department of Biology, College of Natural Sciences, Soonchunhyang University)
Min Kyu Sang
(Korea Native Animal Resources Utilization Convergence Research Institute)
Jie Eun Park
(Korea Native Animal Resources Utilization Convergence Research Institute)
Jun Yang Jeon
(Department of Biology, College of Natural Sciences, Soonchunhyang University)
Chan Eui Hong
(Department of Biology, College of Natural Sciences, Soonchunhyang University)
Yong Tae Kim
(Department of Biology, College of Natural Sciences, Soonchunhyang University)
Hyun Jun Shin
(Department of Biology, College of Natural Sciences, Soonchunhyang University)
Yi Seon Jang
(Department of Biology, College of Natural Sciences, Soonchunhyang University)
Hee Ju Hwang
(Department of Biology, College of Natural Sciences, Soonchunhyang University)
Se Won Kang
(Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology)
So Young Park
(Nakdonggang National Institute of Biological Resources)
Hong Seog Park
(Research Institute, GnC BIO Co.)
Jun Sang Lee
(Korea Native Animal Resources Utilization Convergence Research Institute)
Yeon Soo Han
(Department of Applied Biology, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agriculture and Life Sciences, Chonnam National University)
Yong Seok Lee
(Department of Biology, College of Natural Sciences, Soonchunhyang University)
The Korean Journal of Malacology / The Korean Journal of Malacology, (P)1225-3480;
2023, v.39 no.4, pp.199-205
https://doi.org/10.9710/kjm.2023.39.4.199
Lee,
H., Sang,
M. K., Park,
J. E., Jeon,
J. Y., Hong,
C. E., Kim,
Y. T., Shin,
H. J., Jang,
Y. S., Hwang,
H. J., Kang,
S. W., Park,
S. Y., Park,
H. S., Lee,
J. S., Han,
Y. S., &
Lee,
Y.
S.
(2023). 염주알다슬기 (Koreanomelania nodifila) 의 Arginine Kinase 유전자를 이용한 분자계통학적 분석. , 39(4), 199-205, https://doi.org/10.9710/kjm.2023.39.4.199
Abstract
Arginine kinases (AK) are known to be widely distributed in various invertebrates. It has an essential role in cellular energy metabolism. It regulates energy regulation and homeostasis in response to external stress. AK gene is used as a marker in invertebrate phylogenetic analysis. In this study, we verified whether AK can be used in molecular phylogenetic analysis. We sequenced Koreanomelania nodifila cDNA library using Illumina Hiseq 2000. The AK gene of K. nodifila was sequenced using the BLASTx program and PANM DB (v5.1). AK gene was used as a marker to molecular phylogenetic using the MEGA 11 maximum likelihood method. To analyze the 2D structure of the AK gene, the 2D structure was predicted using PSIPRED (v4.0). As a result, the AK gene ORF of K. nodifila is a total of 352 amino acids and AK gene is considered to be highly useful for molecular phylogenetic analysis of Mollusca.
- keywords
-
Koreanomelania nodifila,
Arginine Kinase,
phylogenetic tree
- Received
- 2023-12-16
- Revised
- 2023-12-26
- Accepted
- 2023-12-31
- Published
- 2023-12-31
ISSN : 1225-3480
