Determination of Putrescine, Spermidine and Spermine in urine by HPLC
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1997, v.10 no.6, pp.445-452
Shin, Tae-Yong
Lee, Seok-Bong
Kim, Hyung-Min
Shin,
T., Lee,
S., &
Kim,
H.
(1997). Determination of Putrescine, Spermidine and Spermine in urine by HPLC. , 10(6), 445-452.
Abstract
A simple and sensitive high performance liquid chromatography for analysis of Putrescine, Spermidine and Spermine in urine is described. The benzoyl chloride derivatives of Putrescine, Spermidine and Spermine are separated on a ${\mu}$ Bondapak $C_{18}$ reverse-phase column with methanol/$H_2O$(50/50v/v) as the mobile phase. The results show that the mean level of polyamines in uterine cancer patients urine is much higher than in normal human urine.
- keywords
-
HPLC analysis,
Putrescine,
Spermidine,
Spermine,
Benzoyl chloride