Determination of Ginseng Saponins by Reversed-Phase High Performance Liquid Chromatography
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1998, v.11 no.6, pp.436-439
Jeong, Seung-Il
Kim, Choen-Suk
Lee, No-Woon
Choi, Kang-Ju
Lee, Yong-Gu
Kim, Il-Kwang
Jeong,
S., Kim,
C., Lee,
N., Choi,
K., Lee,
Y., &
Kim,
I.
(1998). Determination of Ginseng Saponins by Reversed-Phase High Performance Liquid Chromatography. , 11(6), 436-439.
Abstract
Ginseng saponins were analysed using reversed-phase high performance liquid chromatography with several columns. The optimum conditions were as following : reverse phase column; Novapak $C_{18}$ ODS column ($3.9mm{\times}150mm$, $5{\mu}m$), acetonitrile/water binary mobile phase gradient controller system, solvent flow rate; 1.5 mL/min, and UV (203 nm) detector. The complete separation of ginsenoside $Rb_1$, $Rb_2$, Rc, Rd, Re, Rf and $Rg_1$ was achieved within 50 min. The regression coefficients of the calibration curves for seven ginsenosides were 0.98~0.99.
- keywords
-
ginsenoside,
saponin,
reverse-phase high performance liquid chromatography