Determination of Glutathione in Biological Samples by Ion-pairing HPLC/FLD

Yoo, Jeong-Yeon; 유정연; Lee, Kyoung-Ok; 이경옥; Shin, Ho-Sang; 신호상

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P-ISSN1225-0163
E-ISSN2288-8985
SCOPUS, ESCI, KCI

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P-ISSN 1225-0163
E-ISSN 2288-8985

e-Submission

Vol.12, No.1

PubReader Citation

Determination of Glutathione in Biological Samples by Ion-pairing HPLC/FLD

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985

1999, v.12 no.1, pp.28-33

Yoo, Jeong-Yeon
Lee, Kyoung-Ok
Shin, Ho-Sang

Yoo, J., Lee, K., & Shin, H. (1999). Determination of Glutathione in Biological Samples by Ion-pairing HPLC/FLD. , 12(1), 28-33.

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Abstract

Glutathione(GSH) in biological samples was determined by high performance liquid chromatographic(HPLC) method with fluorescence detector(FLD) after monobromobimane(MBB) or 4-fluoro-7-sulfobenzofurazan(SBD-F) derivatization. The detection limit of $0.03{\mu}g/mL$ was obtained after MBB derivatization, derivative of MBB was about 200 times more sensitive than that of SBD-F. N-acetylcysteine was used as internal standard and tetrabutylammonium ion as counter ion for better separation. The determination by ion-pairing chromatography after MBB derivatization was characterized by linearity in the range between $0.08{\sim}8.33{\mu}g/mL$ with a good correlation coefficient of 0.998. By precision test appeared relative standard deviation at less than 5% at three different concentrations. This method can be used for the analysis of GSH in plasma and tissue.