Analysis of the Metabolites of 1,2,4-Trimethylbenzene by Capillary Electrophoresis

Kang, Jong-Seong; 강종성; Hong, Cheong-Hee; 홍정희; Lim, Jeong-Mi; 임정미; Lee, Yong-Moon; 이용문; Jang, Jae-Yeon; 장재연

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P-ISSN1225-0163
E-ISSN2288-8985
SCOPUS, ESCI, KCI

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P-ISSN 1225-0163
E-ISSN 2288-8985

e-Submission

Vol.12, No.4

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Analysis of the Metabolites of 1,2,4-Trimethylbenzene by Capillary Electrophoresis

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985

1999, v.12 no.4, pp.326-331

Kang, Jong-Seong
Hong, Cheong-Hee
Lim, Jeong-Mi
Lee, Yong-Moon
Jang, Jae-Yeon

Kang, J., Hong, C., Lim, J., Lee, Y., & Jang, J. (1999). Analysis of the Metabolites of 1,2,4-Trimethylbenzene by Capillary Electrophoresis. , 12(4), 326-331.

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Abstract

The metabolites of 1,2,4-trimethylbenzene (TMB) were synthesized and determined by capillary electrophoresis (CE). The optimum conditions of CE for the separation and determination of 3,4-, 2,4-, 2,5-dimethylbenzoic acid and 3,4-, 2,4-, 2,5-dimethylhippuric acid from the rat urine were as following: the fused silica capillary($75{\mu}m$ i.d. ${\times}$ 36 cm length, 29 cm to detector) was used and kept at $15^{\circ}C$. The applied voltage was 10㎸ and compounds were detected at UV 210 mnm and 254 nm. The running electrolyte was 0.1 M phosphate buffer (pH 7) containing 15 mM of ${\beta}-CD$ and 3% of 2-propanol. The relative amount of the metabolite of 1,2,4-TMB in the rat urine was 56.7% of 3,4-isomer, 30.5% of 2,4-isomer and 12.8% of 2,5-isomer. This method can be applied to the analysis of TMB-metabolites in human urine.