- P-ISSN 1225-0163
- E-ISSN 2288-8985
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
The Expression and Functional Analysis of Recombinant Alcohol Dehydrogenase
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1999, v.12 no.6, pp.565-570
Kong,
K., Shim,
E., Park,
H., Kim,
E., Cho,
S., Park,
S., &
Kim,
Y.
(1999). The Expression and Functional Analysis of Recombinant Alcohol Dehydrogenase. , 12(6), 565-570.
Abstract
The alcohol dehydrogenase (ADH) gene from Bacillus stearothermopilus was amplified by the polymerase chain reaction. The amplified DNA was inserted into the expression vector pGEX-KG, and expressed it as a fusion protein with glutathione S-transferase (GST) in E. coli. The recombinant ADH was produced by induction with 1 mM isopropyl-
- keywords
- alcohol dehydrogenase, Bacillus stearothermopilus, expression in E. coli
- Downloaded
- Viewed
- 0KCI Citations
- 0WOS Citations
Other articles from this issue
- Rates and Mechanism of Adsorption of Transition Metal Ions on Polystyrene Resins Supported Diethylenetriamine
- Development of Oxygen Sensor for the Oxygen Concentration Measuring of Air-fuel Ratio Measuring System
- A Study on the Coagulation Efficiencies of Some Organics by Aluminum Based Coagulants
- Studies on Determination of trace amount of Se in Volcanic Rocks by Atomic Absorption Spectrophotometry
- Modification of Hydrogen Determinator for Total Hydrogen Analysis in Irradiated Zircaloy Cladding Tube
- Sythesis and Characterization of Transition Metal(II) Complexes with <TEX>$NOTDH_2$</TEX> Schiff Base
- Properties of Polyacene Anode Derived from Phenolic Resin
- Characterization and Synthesis of Titanium (IV) Isopropoxide Derivatives
- Ion-exchange Separation and Spectrophotometric Determination of Trace Amount of Aluminium with Thorinin the Presence of Triton X-100
- Development of Capillary Columns for Ion Chromatography
- A Study on Determination of Iodine in Serum, Fresh Milk, and Feed Additive by Inductively Coupled Plasma-Mass Spectrometry
- Detection of Major Mycotoxins from Contaminated Cereals (Wheat, Soybean and Corn)
- A Study on the PCDDs/PCDFs Contents in the Flue Gas of Muncipal Solid Waste Incinerator(III) -Emission Concentration Varying the Combustion Temperature-
- Analysis of Toxic-PCBs in Sediment by Isotope Dilution HRGC/HRMS
- A Study on the Dose Analysis of Pottery Shards by Thermoluminescence Dating Method
- The Expression and Functional Analysis of Recombinant Alcohol Dehydrogenase
- Simultaneous Analysis of Several Non-Steroidal Anti-Inflammatory Drugs in Urine by Gas Chromatograph/Negative Chemical Ionization-Mass Spectrometry
- Analysis of Blood Toluene and Butane in Death Cases of Inhalant Abusers
- Quantitative Analysis of Nitrate and Nitrite in Rain, Milk and Infant Formula using Ion Chromatography
- Utilization of Chemical Shift Tensor on Solid State Nuclear Magnetic Resonance (It's Theoretical Approach and Application to Structure Analysis)
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.