Determination of Optimum pH of Enzyme by Electrochemical Method

Yoon, Kil-Joong; 윤길중; Lee, Beom-Gyu; 이범규; Kwon, Hyo-Shik; 권효식

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P-ISSN1225-0163
E-ISSN2288-8985
SCOPUS, ESCI, KCI

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P-ISSN 1225-0163
E-ISSN 2288-8985

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Vol.13, No.1

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Determination of Optimum pH of Enzyme by Electrochemical Method

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985

2000, v.13 no.1, pp.41-48

Yoon, Kil-Joong
Lee, Beom-Gyu
Kwon, Hyo-Shik

Yoon, K., Lee, B., & Kwon, H. (2000). Determination of Optimum pH of Enzyme by Electrochemical Method. , 13(1), 41-48.

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Abstract

A biosensor for the determination of hydrogen peroxide was constructed by immobilizing of porcine small instestinal tissue in a plain carbon paste, and the effect of varying the $H_2O_2$ concentration and pH on the rate of catalytic reaction was evaluated. For the mathematical simplicity, no mediator was added. Electrochemical properties and the maximal rate could be derived from the quantitative analysis of the observed phenomena related to the electrode reaction. Also, pH dependence of the Michaelis constant enabled to calculate various thermodynamic parameters and subsequently to design a electrochemical method to determine the optimum pH of enzyme.