Rapid Quantitative Analysis of Vancomycin in Human Plasma and Urine Using LC-MS/MS

김호현; 노형진; 한상범

오늘 하루 그만보기

P-ISSN1225-0163
E-ISSN2288-8985
SCOPUS, ESCI, KCI

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P-ISSN 1225-0163
E-ISSN 2288-8985

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Vol.15, No.5

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Rapid Quantitative Analysis of Vancomycin in Human Plasma and Urine Using LC-MS/MS

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985

2002, v.15 no.5, pp.410-416

& (2002). Rapid Quantitative Analysis of Vancomycin in Human Plasma and Urine Using LC-MS/MS. , 15(5), 410-416.

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Abstract

In this study, a new quantitative analytical method has been developed for the rapid determination of vancomycin in human plasma and urine using liquid chromatography/tandem mass spectrometry (LC - MS/MS). Chromatography was carried out on a $C_{18}$ XTerra MS column ($2.1{\times}30mm$) with a particle size of $3.5{\mu}m$. The mobile phase was 0.25% formic acid in 10% acetonitrile and the flow rate was $250{\mu}L/min$. Vancomycin and caffeine (internal standard) were detected by MS/MS using multiple reaction monitoring (MRM). Vancomycin gives a predominant doubly protonated precursor molecule ($[M+2H]^{2+}$) at m/z 725.0 and a corresponding product ion of m/z 100.0. Detection of vancomycin was good, accurate and precise, with a limit of detection of 1 nM in plasma. The calibration curves for vancomycin in human plasma was linear in a concentration range of $0.01{\mu}M$ - $100{\mu}M$ for plasma. This method has been successfully applied to determine the concentration of vancomycin in human plasma and urine from pharmacokinetic study and relative studies.