Development of electrochemical biosensor for determination of galactose

남학현; 박갑수; 조순삼; 권덕; 이재선; 차근식

오늘 하루 그만보기

P-ISSN1225-0163
E-ISSN2288-8985
SCOPUS, ESCI, KCI

Home

Browse Articles

Article Detail

Home > Article Detail

P-ISSN 1225-0163
E-ISSN 2288-8985

e-Submission

Vol.20, No.5

PubReader PDF Citation

Development of electrochemical biosensor for determination of galactose

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985

2007, v.20 no.5, pp.393-399

& (2007). Development of electrochemical biosensor for determination of galactose. , 20(5), 393-399.

copy

Abstract

In principle, the blood galactose level may be determined conveniently with a strip-type biosensor similar to that for glucose. In this study, we describe the development of a disposable galactose biosensor strip for point-of-care testing. The sensor strip is constructed with screen-printed carbon paste electrode (SPCE) and sample amount (< $100{\mu}L$). The developed strip the galactose level in less than 90 s using bienzymatic system of galactose oxidase (GAO) and horseradish peroxidase (HRP). The effects of pH, mediator (1,1-ferrocenedimethanol) concentration, ratio of enzymes, and applied potential were determined preliminarily with glassy carbon electrodes, and optimized further with the strip-type electrodes. The sensor exhibits linear response in the range of $0{\sim}400{\mu}M$ ($r^2$

keywords: galactose biosensor, galactosemia, screen-printed carbon paste electrodes, point-of-care testing, galactose oxidase, horseradish peroxidase