The distribution of <TEX>$^{14}C$</TEX>-warfarin and the purification of hepatic microsome induced isozymes with coumarin

Park, Sung-Woo; 박성우; Kim, Eun-Ho; 김은호; Min, Ji-Sook; 민지숙; You, Jae-Hoon; 유재훈; Lee, Hee-Sung; 이희성; Seo, Bae-Seck; 서배석; Han, Wan-Soo; 한완수

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P-ISSN1225-0163
E-ISSN2288-8985
SCOPUS, ESCI, KCI

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P-ISSN 1225-0163
E-ISSN 2288-8985

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Vol.5, No.1

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The distribution of $^{14}C$-warfarin and the purification of hepatic microsome induced isozymes with coumarin

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985

1992, v.5 no.1, pp.83-90

Park, Sung-Woo
Kim, Eun-Ho
Min, Ji-Sook
You, Jae-Hoon
Lee, Hee-Sung
Seo, Bae-Seck
Han, Wan-Soo

Park, S., Kim, E., Min, J., You, J., Lee, H., Seo, B., & Han, W. (1992). The distribution of <TEX>$^{14}C$</TEX>-warfarin and the purification of hepatic microsome induced isozymes with coumarin, 5(1), 83-90.

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Abstract

The $^{14}C$-warfarin used as rodenticids was identified from various organs of sprague dawley with scintillation counter. And the cytochrome p-450 which was induced by coumarin derivatives was identified with electrophoresis. The distributions of $^{14}C$-warfarin after $14.8{\mu}Ci/kg$ oral application at each organ was as follows; urine-7.5%, blood-0.44%, feces-0.9%, liver-0.66%, lung-0.86%, kidney-0.8%, heart-0.43% and spreen-0.33% after 24hrs. The cytochrome p-450 was purified by Octyl Sepharose CL-4B hydrophobic chromatography and isozymes were 50.8 Kd in control group, 53.3 Kd and 55.2 Kd in coumarin pretreated group and 50.8 Kd, 54.6 Kd and 57.7 Kd in warfarin pretreated group.