A Study on the Analysis of Methylprednisolone Acetate and its Metabolites in Rat Urine by LC/MS

Park, Song-Ja; 박송자; Pyo, Hee Soo; 표희수; Kim, Yun Je; 김연제; Park, Seong Soo; 박성수; Park, Jongsei; 박종세

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P-ISSN1225-0163
E-ISSN2288-8985
SCOPUS, ESCI, KCI

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P-ISSN 1225-0163
E-ISSN 2288-8985

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Vol.8, No.2

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A Study on the Analysis of Methylprednisolone Acetate and its Metabolites in Rat Urine by LC/MS

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985

1995, v.8 no.2, pp.139-159

Park, Song-Ja
Pyo, Hee Soo
Kim, Yun Je
Park, Seong Soo
Park, Jongsei

Park, S., Pyo, H. S., Kim, Y. J., Park, S. S., & Park, J. (1995). A Study on the Analysis of Methylprednisolone Acetate and its Metabolites in Rat Urine by LC/MS. , 8(2), 139-159.

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Abstract

Positive ion mass spectra of some corticosteroids were obtained by using liquid chromatography-mass spectrometry(LC-MS). The base peak of each compound showed the protonated molecular ion [$MH^+$], ammonium adduct ion [${MNH_4}^+$] or [$MH^+-60$] ion according to its chemical structure and other characteristic mass ions were [$MH^+-18$], [${MNH_4}^+-18$] and so on. Several rat urinary metabolites of methylprednisolone acetate after the oral administration were detected by the thermospray LC-MS. The identified major metabolites were 20-hydroxymethylprednisolone(20-HMP), methylprednisolone(MP) and methylprednisone(11-KMP), which were supposed to be formed by deacetylation at the position of C-21, reduction at C-20, oxidation at C-11, or due to the bond cleavage between C-17 and C-20.