Determination of <TEX>$Ca^{2+}$</TEX> by Fiber Optic Fluorosensor Based on the Conformational Change of the Protein Calmodulin

Ri, Chang-Seop; 이창섭; Yang, Seung Tae; 양승태

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P-ISSN1225-0163
E-ISSN2288-8985
SCOPUS, ESCI, KCI

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P-ISSN 1225-0163
E-ISSN 2288-8985

e-Submission

Vol.8, No.3

PubReader Citation

Determination of $Ca^{2+}$ by Fiber Optic Fluorosensor Based on the Conformational Change of the Protein Calmodulin

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985

1995, v.8 no.3, pp.221-227

Ri, Chang-Seop
Yang, Seung Tae

Ri, C., & Yang, S. T. (1995). Determination of <TEX>$Ca^{2+}$</TEX> by Fiber Optic Fluorosensor Based on the Conformational Change of the Protein Calmodulin. , 8(3), 221-227.

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Abstract

The fiber optic fluorosensor that shows a specific selectivity for calcium ion is studied. This sensor employs protein Calmodulin(CaM) which forms a fluorescent chelate with $Ca^{2+}$. A dialysis membrane is used to entrap a fluorescein isothiocyanate-labeled CaM solution at the common end of a bifurcated fiber optic bundle. The sensing mechanism of this sensor is based on the shifts in the fluorescence spectrum of metal-calmodulin complexes which FCaM forms a chelate with $Ca^{2+}$. Upon binding with $Ca^{2+}$, CaM undergoes a conformational change which induces a change in the fluorescence of FCaM. This change in fluorescence signal which is measured by photomultiflier tube is related to the concentration of $Ca^{2+}$ for calibration curve. Detection limit for $Ca^{2+}$ and the interference effects by $Mg^{2+}$, $Eu^{3+}$ and $La^{3+}$ for this sensor are studied. Response time and life time for this fluorosensor are also investigated.