바로가기메뉴

본문 바로가기 주메뉴 바로가기
 
 

logo

메뉴
search

ISSN : 1225-3480

논문 상세

이전 다음
논문 투고

26권 1호

Ultrastructures of Germ Cells and the Accessory Cells During Spermatogenesis in Male Gomphina veneriformis (Bivalvia: Veneridae) on the East Sea of Korea

Ultrastructures of Germ Cells and the Accessory Cells During Spermatogenesis in Male Gomphina veneriformis (Bivalvia: Veneridae) on the East Sea of Korea

Chung, Ee-Yung (Korea Marine Environment & Ecosystem Institute, Dive Korea)
Chung, Chang-Ho (CMAS Kore)
Kim, Jin-Hee (Korea Inter-University Unstitute of Ocean Science, Pukyong National University)
Park, Sung-Woo (Department of Aquatic Life Medicine, Kunsan National University)
Park, Kwan-Ha (Department of Aquatic Life Medicine, Kunsan National University)
한국패류학회지 / The Korean Journal of Malacology, (P)1225-3480;
2010, v.26 no.1, pp.51-62
Chung, Ee-Yung, Chung, Chang-Ho, Kim, Jin-Hee, Park, Sung-Woo, & Park, Kwan-Ha. (2010). Ultrastructures of Germ Cells and the Accessory Cells During Spermatogenesis in Male Gomphina veneriformis (Bivalvia: Veneridae) on the East Sea of Korea. , 26(1), 51-62.

Abstract

The ultrastructures of germ cells and the accessory cells during spermatogenesis and mature sperm ultrastructure in male Gomphina veneriformis, which was collected on the coastal waters of Yangyang, East Sea of Korea, were investigated by transmission electron microscope observations. The morphology of the spermatozoon has a primitive type and is similar to those of other bivalves in that it contains a short midpiece with four mitochondria surrounding the centrioles. Accessory cells are observed to be connected to adjacent germ cells, they contain a large quantity of glycogen particles and lipid droplets in the cytoplasm. Therefore, it is assumed that they are involved in the supplying of the nutrients for germ cell development, while any phenomena associated with phagocytosis of undischarged, residual sperms by lysosomes in the cytoplasm of the accessory cells after spawning was not observed in this study. The morphologies of the sperm nucleus type and the acrosome shape of this species have a cylindrical and modified long cone shape, respectively. In particular, the axial filaments in the lumen of the acrosome, and subacrosomal granular materials are observed in the subacrosomal space between the anterior nuclear fossa and the beginning part of axial filaments in the acrosome. The spermatozoon is approximately $50-55{\mu}m$ in length including a long sperm nucleus (about $7.80{\mu}m$ in length), an acrosome (about $1.13{\mu}m$ in length) and tail flagellum ($40-45{\mu}m$). The axoneme of the sperm tail flagellum consists of nine pairs of microtubules at the periphery and a pair at the center. The axoneme of the sperm tail shows a 9+2 structure. Some charateristics of sperm morphology of this species in the family Veneridae are (1) acrosomal morphology, (2) the number of mitochondria in the midpiece of the sperm,. The axial filament appears in the acrosome as one of characteristics seen in several species of the family Veneridae in the subclass heterodonta, unlikely the subclass pteriomorphia containing axial rod instead of the axial filament. As some characteristics of the acrosome structures, the peripheral parts of two basal rings show electron opaque part (region), while the apex part of the acrosome shows electron lucent part (region). These charateristics belong to the family Veneridae in the subclass heterodonta, unlikely a characteristic of the subclass pteriomorphia showing all part of the acrosome being composed of electron opaque part (region). Therefore, it is easy to distinguish the families or the subclasses by the acrosome structures. The number of mitochondria in the midpiece of the sperm of this species are four, as one of common characteristics appeared in most species in the family Veneridae.

keywords
Gomphina veneriformis, spermatogenesis, germ cell, accessory cell

참고문헌

1.

(1991). . Journal of Medical and Applied Malacology, 3, 125-136.

2.

Sousa. (1989). . Gamete Research, 24(4), 393-401. 10.1002/mrd.1120240406.

3.

(1994). . Marine Biology, 120, 41-47.

4.

5.

6.

Niijima, Lumiko;Dan, Jean. (1965). The Acrosome Reaction in Mytilus edulis. I. Fine Structure of the Intact Acrosome. The Journal of Cell Biology, 25(2), 243-248. 10.1083/jcb.25.2.243.

7.

박정준;이정용;이정식;장영진. (2003). 대복 (Gomphina veneriformis)의 생식소 발달과 생식주기. Korean Journal of Fisheries and Aquatic Sciences, 36(4), 352-357. 10.5657/kfas.2003.36.4.352.

8.

(1987). . Mar. Biol., 96, 919-528.

9.

(1974). . Cell Tissue Research, 150, 291-297.

10.

(1979). . Malacological Review, 12, 1-20.

11.

12.

(1999). . Journal of Korean Fisheries Society, 32, 198-203.

13.

Komaru, A.;Konishi, K.. (1996). Ultrastructure of biflagellate spermatozoa in the freshwater clam, Corbicula leana (Prime). Invertebrate Reproduction & Development, 29(3), 193-197. 10.1080/07924259.1996.9672513.

14.

(1985). . Scientific Report of Faculty of Education, Gunma University, 34, 33-153.

15.

Komaru, Akira;Konishi, Kooichi;Nakayama, Ichiro;Kobayashi, Takanori;Sakai, Harumi;Kawamura. (1997). Hermaphroditic Freshwater Clams in the Genus Corbicula Produce Non-Reductional Spermatozoa with Somatic DNA Content. Biological Bulletin, 193(3), 320-323. 10.2307/1542934.

16.

17.

HEALY. (1995). . Zoologica Scripta, 24(1), 21-28. 10.1111/j.1463-6409.1995.tb00472.x.

18.

Franz占�n. (1983). . Gamete Research, 7(3), 199-214. 10.1002/mrd.1120070302.

19.

De Gaulejac, B.;Henry, M.;Vicente, N.. (1995). An ultrastructural study of gametogenesis of the marine bivalve Pinna nobilis (Linnaeus, 1758). II. Spermatogenesis. Journal of Molluscan Studies, 61(3), 393-403. 10.1093/mollus/61.3.393.

20.

Healy. (1989). . Marine Biology, 103(1), 75-85. 10.1007/BF00391066.

21.

Hodgson. (1986). . Gamete Research, 15(2), 123-135. 10.1002/mrd.1120150204.

22.

23.

Eckelbarger, K. J.;Davis, C. V.. (1996). Ultrastructure of the gonad and gametogenesis in the eastern oyster, Crassostrea viriginica. II. Testis and spermatogenesis. Marine Biology, 127(1), 89-96. 10.1007/BF00993648.

24.

DORANGE. (1989). . Invertebrate Reproduction & Development, 15(2), 109-117. 10.1080/07924259.1989.9672031.

25.

(1986). . Tsitologiia, 28, 1069-1074.

26.

Eckelbarger. (1990). . Journal of Morphology, 205(1), 63-75. 10.1002/jmor.1052050107.

27.

Daniels, E W;Longwell, A C;McNiff, J M;Wolfgang, R W. (1971). Ultrastructure of spermatozoa from the American oyster Crassostrea virginica.. Transactions of the American Microscopical Society, 90(3), 275-282. 10.2307/3225187.

28.

(1998). . Development and Reproduction, 2(1), 101-109.

29.

(2005). . Korean Journal of Malacology, 21, 95-105.

30.

(2000). . Malacologia, 42, 149-163.

31.

(2006). . The Korean Journal of Malacology, 22, 27-38.

32.

33.

(1985). . South Africa Journal of Zoology, 20, 5-9.

34.

Chung, Ee-Yung;Kim, Eun-Jong;Park, Gab-Man. (2007). Spermatogenesis and Sexual Maturation in Male Mactra chinensis (Bivalvia: Mactridae) of Korea. Integrative Biosciences, 11(2), 227-234. 10.1080/17386357.2007.9647339.

35.

(1999). . Yellow Sea, 5, 51-58.

36.

(2002). . Korean Journal of Electron Microscopy, 32, 303-310.

logo

논문 투고
OA 정책