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ISSN : 1225-3480

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26권 1호

TBTCl 독성에 의한 대복, Gomphina veneriformis 소화선의 미세구조적 변화 및 지방갈색소 축적

Ultrastructural Changes in digestive gland and Lipofuscin Accumulation of the Equilateral Venus, Gomphina veneriformis (Bivalvia: Veneridae) on Tributyltin chloride (TBTCl) Toxicity

박정준 (국립수산과학원 병리연구과)
이정식 (전남대학교 수산해양대학 수산생명의학과)
한국패류학회지 / The Korean Journal of Malacology, (P)1225-3480;
2010, v.26 no.1, pp.63-78
박정준, & 이정식. (2010). TBTCl 독성에 의한 대복, Gomphina veneriformis 소화선의 미세구조적 변화 및 지방갈색소 축적. , 26(1), 63-78.

초록

TBTCl에 36주 동안 노출된 대복, Gomphina veneriformis 소화선의 미세구조적 변화와 지방갈색소의 축적을 관찰한 결과 외막상피층의 경우 점액세포 감소, 섬모 탈락 및 선조연의 부분적인 소실이 관찰되었다. 소화선세관에서는 저농도의 개체들에서 소화선세관 내강에 소화효소의 양이 증가하였지만 농도 의존적으로 소화선세관의 세포들이 입방형 또는 편평형으로 변하여 세포층의 두께가 얇아지며, 부분적으로 파괴가 관찰되었다. 이러한 광학현미경적 증상들을 자현미경으로 관찰한 결과 내장낭 외막 상피세포와 소화선세관을 둘러싸고 있는 근섬유사이에는 다수의 글리코겐 과립들이 관찰되었고, 호염기성 세포들에서는 조면소포체의 파괴와 세포의 괴사로 인하여 핵의 응축 및 핵막의 파괴가 관찰되었다. 이러한 소화선세관 세포들의 변화로 세포층은 고농도로 대조구와 유의적인 차이를 보이며, 두께가 감소하였다 (P < 0.05). Long Ziehl Neelsen 염 결과, 소화선세관의 세포에서 자주색으로 관찰되는 지방갈색소는 대조구와 유의적인 차이를 보이며 증가하였지만 (P < 0.05), 노출이 진행될수록 소화선세관 세포들이 파괴됨에 따라 그 축적도 감소하였다. 따라서 본 연구 결과 TBT는 대복 소화선세관 세포들의 미세구조를 파괴함에 따라서 이들 세포들이 가지는 소화기능을 억제하는 것으로 생각되어지며, 농도 의존적으로 지방갈색소의 축적이 높아지기 때문에 지방 갈색소는 TBT의 오염정도를 파악하기 위한 biomarker로서 충분히 이용 가능한 것으로 생각된다.

keywords
Gomphina veneriformis, ultrastructural change, digestive gland, lipofuscin, TBT

Abstract

The purpose of this study was to investigate the effect of tributyltin (TBT) on histopathological and ultrastructural changes in the digestive gland structure of the equilateral venus, Gomphina veneriformis. Experimental period was 36 weeks. Experimental groups consist of control condition and 3 TBTCl exposure conditions (0.4, 0.6, $0.8\;TBTC\;{\mu}g\;L^{-1}$). Outer envelop of the visceral mass of G. veneriformis exposed to TBTCl was observed disappearance of microvilli and cilia, decrease of mucous cell and partially destruction of epithelium. In the digestive gland showed an increase of number of hemocyte and mucopolysaccaride near the digestive tubule at early time of the exposure. Especially, in $0.8\;TBTC\;{\mu}g\;L^{-1}$ group, collapse of digestive tubule with modification of epithelium was observed. TEM observation revealed the numerous glycogen granules in epithelium of the outer envelop and connective tissue. In the ciliated cell of the primary duct formed the cilia in cytoplasm. Basophilic cell was observed destruction of the rough endoplasmic reticulum and mitochondria. Also, nucleus in the epithelium of the digestive tubule was disappeared heterochromatin and nucleolus, and condense. As the concentration of TBTCl increased, the accumulation of lipofucin increased in the digestive gland, but the collapse of digestive tubule induced a decrease of accumulation of lipofuscin.

keywords
Gomphina veneriformis, ultrastructural change, digestive gland, lipofuscin, TBT

참고문헌

1.

Siah, A.;Pellerin, J.;Amiard, J.-C.;Pelletier, E.;Viglino, L.. (2003). Delayed gametogenesis and progesterone levels in soft-shell clams (Mya arenaria) in relation to in situ contamination to organotins and heavy metals in the St. Lawrence River (Canada). Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 135(2), 145-156. 10.1016/S1532-0456(03)00085-1.

2.

(1971). . Proc. Malacol. Soc. Lond., 39, 377-378.

3.

4.

5.

Byrne, P.A.;Halloran, J.O.. (1999). Aspects of Assaying Sediment Toxicity in Irish Estuarine Ecosystems. Marine Pollution Bulletin, 39(1), 97-105. 10.1016/S0025-326X(99)00121-6.

6.

Adelman. (1990). . Environmental Science & Technology, 24(7), 1027-1032. 10.1021/es00077a012.

7.

Alzieu, C.L.;Sanjuan, J.;Deltreil, J.P.;Borel, M.. (1986). Tin contamination in Arcachon Bay: Effects on oyster shell anomalies. Marine Pollution Bulletin, 17(11), 494-498. 10.1016/0025-326X(86)90636-3.

8.

9.

Santos, M.M.;Vieira, N.;Santos, A.M.. (2000). Imposex in the Dogwhelk Nucella lapillus (L.) along the Portuguese Coast. Marine Pollution Bulletin, 40(7), 643-646. 10.1016/S0025-326X(00)00017-5.

10.

Santos, M.M.;Ten Hallers-Tjabbes, C.C.;Santos, A.M.;Vieira, N.. (2002). Imposex in Nucella lapillus, a bioindicator for TBT contamination: re-survey along the Portuguese coast to monitor the effectiveness of EU regulation. Journal of Sea Research, 48(3), 217-223. 10.1016/S1385-1101(02)00166-1.

11.

12.

(1970). . Porc. Malacol. Scotland., 39, 231-233.

13.

Regoli. (1992). . Marine Ecology Progress Series, 84, 63-69. 10.3354/meps084063.

14.

Regoli, F.;Orlando, E.. (1994). Accumulation and subcellular distribution of metals (Cu, Fe, Mn, Pb and Zn) in the Mediterranean mussel Mytilus galloprovincialis during a field transplant experiment. Marine Pollution Bulletin, 28(10), 592-600. 10.1016/0025-326X(94)90360-3.

15.

(1996). . J. Zool., 74, 1964-1973.

16.

Quinn, B.;Gagne, F.;Costello, M.;McKenzie, C.;Wilson, J.;Mothersill, C.. (2004). The endocrine disrupting effect of municipal effluent on the zebra mussel (Dreissena polymorpha). Aquatic Toxicology, 66(3), 279-292. 10.1016/j.aquatox.2003.10.007.

17.

(1983). . Aquat. Toxicol 3, 3, 301-311.

18.

Otludil, B.;Cengiz, E.I.;Yildirim, M.Z.;Unver, O.;Unlu, E.. (2004). The effects of endosulfan on the great ramshorn snail Planorbarius corneus (Gastropoda, Pulmonata): a histopathological study. Chemosphere, 56(7), 707-716. 10.1016/j.chemosphere.2004.04.027.

19.

(1972). . Sci. Prog. Oxf., 60, 299-318.

20.

21.

(1993). . Amer. Malac. Bull., 10, 1-24.

22.

(2007). . J. Kor. Fish. Soc., 40, 16-23.

23.

24.

(1983). . Malacologia, 23, 375-396.

25.

(1990). . Histochem, 22, 279-287.

26.

Moore, M.N.;Allen, J.I.;Somerfield, P.J.. (2006). Autophagy: Role in surviving environmental stress. Marine Environmental Research, 62(suppl1), S420-S425. 10.1016/j.marenvres.2006.04.055.

27.

Lomovasky. (2002). . Journal of Experimental Marine Biology and Ecology, 276(1-2), 83-94. 10.1016/S0022-0981(02)00240-X.

28.

(1997). . Indian Journal of Marine Sciences, 26, 64-67.

29.

Moore. (1988). . Marine Ecology Progress Series, 46, 81-89. 10.3354/meps046081.

30.

(1997). . Comp. Biochem. Physiol. C, 118, 11-18.

31.

Hole. (1993). . Marine Ecology Progress Series, 94, 175-179. 10.3354/meps094175.

32.

Hole. (1995). . Marine Ecology Progress Series, 122, 173-178. 10.3354/meps122173.

33.

Currie, D.R.;Sorokin, S.J.. (1994). Evaluating the effects of reserve closure on algae, invertebrate and fish assemblages at a temperate South Australian marine reserve. Journal of the Marine Biological Association of the United Kingdom, 74(4), 651-669. 10.1017/S002531540004772X.

34.

Hinch. (1987). . Canadian Journal of Zoology, 65(10), 2436-2442. 10.1139/z87-368.

35.

Horiguchi, T.;Takiguchi, N.;Cho, H.S.;Kojima, M.;Kaya, M.;Shiraishi, H.;Morita, M.;Hir. (2000). Ovo-testis and disturbed reproductive cycle in the giant abalone, Haliotis madaka: possible linkage with organotin contamination in a site of population decline. Marine Environmental Research, 50(1), 223-229. 10.1016/S0141-1136(00)00071-4.

36.

Gomez-Ariza, J.L.;Giraldez, I.;Morales, E.. (2000). Temporal fluctuations of tributyltin in the bivalve Venerupis decussata at five stations in southwest Spain. Environmental Pollution, 108(2), 279-290. 10.1016/S0269-7491(99)00184-0.

37.

International Review of Cytology Volume 234. (2004). . International Review of Cytology, 234(234), 101-141. 10.1016/S0074-7696(04)34003-9.

38.

HOLE. (1992). . Marine Environmental Research, 34(1-4), 75-80. 10.1016/0141-1136(92)90086-2.

39.

Yamada, H.;Takayanagi, K.. (1992). Bioconcentration and elimination of bis(tributyltin)oxide (TBTO) and triphenyltin chloride (TPTC) in several marine fish species. Water Research, 26(12), 1589-1595. 10.1016/0043-1354(92)90158-Z.

40.

(1998). . Frodskaparrit, 46, 67-80.

41.

Gibbs. (1986). . Journal of the Marine Biological Association of the United Kingdom, 66(04), 767-777. 10.1017/S0025315400048414.

42.

Domouhtsidou, G.P.;Dimitriadis, V.K.. (2001). Lysosomal and lipid alterations in the digestive gland of mussels, Mytilus galloprovincialis (L.) as biomarkers of environmental stress. Environmental Pollution, 115(1), 123-137. 10.1016/S0269-7491(00)00233-5.

43.

VIARENGO. (1990). . Comparative Biochemistry and Physiology Part C: Comparative Pharmacology, 97(1), 37-42. 10.1016/0742-8413(90)90168-9.

44.

45.

(1994). . Arch. Environ. Contam. Toxicol, 24, 338-345.

46.

Viarengo. (1993). . Comparative Biochemistry and Physiology Part C: Comparative Pharmacology, 104(3), 355-372. 10.1016/0742-8413(93)90001-2.

47.

Waldock, M.J.;Thain, J.E.. (1983). Shell thickening in Crassostrea gigas: Organotin antifouling or sediment induced?. Marine Pollution Bulletin, 14(11), 411-415. 10.1016/0025-326X(83)90445-9.

48.

Soto, M.;Cajaraville, M.P.;Marigomez, I.. (1996). Tissue and cell distribution of copper, zinc and cadmium in the mussel, Mytilus galloprovincialis, determined by autometallography. Tissue and Cell, 28(5), 557-568. 10.1016/S0040-8166(96)80058-9.

49.

Stephenson, M.. (1991). A field bioassay approach to determining tributyltin toxicity to oysters in California. Marine Environmental Research, 32(1), 51-59. 10.1016/0141-1136(91)90033-5.

50.

Dimitriadis, V.K.;Domouhtsidou, G.P.;Raftopoulou, E.. (2003). Localization of Hg and Pb in the palps, the digestive gland and the gills in Mytilus galloprovincialis (L.) using autometallography and X-ray microanalysis. Environmental Pollution, 125(3), 345-353. 10.1016/S0269-7491(03)00122-2.

51.

Dubey, S. K.;Roy, U.. (2003). Review: Biodegradation of tributyltins (organotins) by marine bacteria. Applied Organometallic Chemistry, 17(1), 3-8. 10.1002/aoc.394.

52.

Brick, M.;Bolte, M.. (1994). Cytology of the outer penis epithelium of Buccinum undatum (L.) from the arctic region - an observation of the imposex phenomenon. Helgol채nder Meeresuntersuchungen, 48(1), 123-131. 10.1007/BF02366206.

53.

Sukhotin. (2002). . Marine Ecology Progress Series, 226, 223-234. 10.3354/meps226223.

54.

55.

Byrne. (2001). . Hydrobiologia, 465(1/3), 209-217. 10.1023/A:1014584607501.

56.

Chiavarini, S.;Massanisso, P.;Nicolai, P.;Nobili, C.;Morabito, R.. (2003). Butyltins concentration levels and imposex occurrence in snails from the Sicilian coasts (Italy). Chemosphere, 50(3), 311-319. 10.1016/S0045-6535(02)00601-X.

57.

Da Ros, L.;Nasci, C.;Marigomez, I.;Soto, M.. (2000). Biomarkers and trace metals in the digestive gland of indigenous and transplanted mussels, Mytilus galloprovincialis, in Venice Lagoon, Italy. Marine Environmental Research, 50(1), 417-423. 10.1016/S0141-1136(00)00038-6.

58.

Sole, M.;Morcillo, Y.;Porte, C.. (1998). Imposex in the commercial snail Bolinus brandaris in the northwestern Mediterranean. Environmental Pollution, 99(2), 241-246. 10.1016/S0269-7491(97)00186-3.

59.

de Mora, S. J.;King, N. G.;Miller, M. C.. (1989). Tributyltin and total tin in marine sediments: Profiles and the apparent rate of TBT degradation. Environmental Technology Letters, 10(10), 901-908. 10.1080/09593338909384811.

60.

(2004). . J. Mol. Histol., 35, 501-509.

61.

Brunk, U.T.;Terman, A.. (2002). Lipofuscin: mechanisms of age-related accumulation and influence on cell function. Free Radical Biology and Medicine, 33(5), 611-619. 10.1016/S0891-5849(02)00959-0.

62.

Zorita, I.;Ortiz-Zarragoitia, M.;Soto, M.;Cajaraville, M.P.. (2006). Biomarkers in mussels from a copper site gradient (Visnes, Norway): An integrated biochemical, histochemical and histological study. Aquatic Toxicology, 78(suppl), S109-S116. 10.1016/j.aquatox.2006.02.032.

63.

Folsvik, N.;Berge, J.A.;Brevik, E.M.;Walday, M.. (1999). Quantification of organotin compounds and determination of imposex in populations of dogwhelks (Nucella lapillus) from Norway. Chemosphere, 38(3), 681-691. 10.1016/S0045-6535(98)00202-1.

64.

Krishnakumar, P.K.;Asokan, P.K.;Pillai, V.K.. (1990). Physiological and cellular responses to copper and mercury in the green mussel Perna viridis (Linnaeus). Aquatic Toxicology, 18(3), 163-173. 10.1016/0166-445X(90)90024-J.

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