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ISSN : 1225-3480
The genus Assiminea (Gastropoda: Assimineidae) are amphibious, brackish snails that inhabit moist areas near marine or non-marine water bodies. Here, eleven specimens of Assiminea grayana are collected from river estuaries and the intertidal to supratidal zones of the western and southern seas of the Korean Peninsula. It is the first record of Assiminea grayana J. Fleming, 1828 in the Korean fauna. To verify the species identification, we examined its ecological characteristics, morphological features and DNA barcoding, which consistently supported the existance of A. grayana in Korea. This species has 6-7 slightly swollen whorls, impressive suture, and sharpened apex. The angle of the aperture acute was adapical. The blackish cephalic tentacles form short, rounded lobes each with eyes at the tip, and their gray snout is divided into two parts. Its COI sequence showed 99.7% similarity to those of individuals from the UK and Germany. The ML tree showed a close relationship of A. grayana from Korea and Europe, which formed a sister group with A. hiradoensis and A. estuarina exclusively inhabiting Northeastern Asia. It is likely to imply that A. grayana might be originated from Northestern Asia. Further studies integrating morphological and molecular data are essential for clarifying the taxonomic status and migration histories of A. grayana and related species.
Research on Korean molluscs began with the recording of 18 species of molluscs by A. Adams & L. Reeve (1850). Currently, a total of 2,037 species of Korean molluscs have been recorded in the National Species List of Korea. In celebration of the 40th anniversary of the founding of The Malacological Society of Korea, this study introduces the history of research on Korean molluscs conducted from 1850 to the present.
The genus Nerita (Gastropoda: Neritidae) inhabits tropical and temperate regions, which is widely distributed in intertidal areas. Recently, it was suggested that Nerita albicilla was divided into the two different species with addition of N. originalis sp. nov. In the previous study (Park et al., 2023), the morphometric data inferred from shell morphological characteristics were first employed to distinguish the two Nerita species from each other. To examine taxonomic significance of shell morphological differences, we have done the normality test of the nine ratios from shell morphology suggested by Park et al. (2023). The result revealed that only six shell morphology ratios (SW/SL, H/SL, H/SW, AL/SW, AW/SW, and AW/SL) possess appropriate normality. Through a bivariate correlation test conducted with the six ratios, we found only four ratio sets of shell morphology have little correlation- (SW/SL, AW/SL), (H/SL, AW/SL), (H/SW, AW/SL), and (AL/SW, AW/SL). With them, we performed the logistic regression and discriminant analyses. The results suggested that (H/SL, AW/SL) has the highest average percentage value (74.7%) in species identification accuracy of the two Nerita species. The present result indicates that the employment of statistical approaches could serve as a viable method in analyzing Nerita shell morphology for taxonomic use.
This study aimed to utilize next-generation sequencing for developing microsatellite markers in Fulvia mutica. The Illumina NovaSeq 6000 System generated a total of 327,584,690 reads, resulting in a sequence length of 49,465,288,190 bp. The de novo assembly yielded an 885 Mb (885,020,821 bp) sequence. In this context, 675,057 contigs longer than 500 bp were identified. Among these, 107,985 contigs containing microsatellite regions were identified, accounting for 15.9%. A total of 105,632 microsatellite loci were found within these 107,985 microsatellite regions. Among these loci, polymorphism information was confirmed at 3,723 (3.5%) microsatellite marker positions. eighty-two primer sets were designed based on the 150 microsatellite loci. As a result, 14 microsatellite loci were selected for estimating population genetic parameters in both wild populations. The mean number of effective alleles was 11, ranging from 7 to 18. The observed heterozygosity (HO) ranged from 0.615 to 0.937, with an average of 0.790, while the expected heterozygosity (HE) ranged from 0.629 to 0.924, also with an average of 0.790. Based on these findings, the compiled panel of 14 microsatellite markers is anticipated to facilitate examining genetic traits within the Fulvia mutica population in Korea. The results of the analysis of genetic diversity and gene relationships in Fulvia mutica are expected to be crucial data for the management, conservation, and sustainable utilization of genetic resources. Such information is expected to play a pivotal role in developing conservation and management strategies and formulating policies and strategies for sustainable utilization of genetic resources.
The updated PANM database version 6.0 includes approximately 1.3 times more amino acid sequences compared to its previous version. Although it contains only about 3.6% of the sequences compared to the commonly used NCBI-nr database for annotation, PANM 6.0 was specifically constructed using amino acid sequences of genes from Arthropoda, Nematoda, and Mollusca. This allows for reduced annotation time and improved accuracy for genes, genomes, or transcriptome sequences of species belonging to these three phyla. Like previous versions, PANM database version 6 (http://panm.sch.ac.kr/) freely accessible to all researchers and is expected to be useful for researchers studying invertebrates in the future.
