Analysis of Amineptine and its Metabolites in Human Urine by Gas Chromatography/Mass Spectrometry

Lee, Jeong Ae; 이정애; Kim, Younglim; 김영림; Lho, Dong-Seok; 노동석

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P-ISSN1225-0163
E-ISSN2288-8985
SCOPUS, ESCI, KCI

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P-ISSN 1225-0163
E-ISSN 2288-8985

e-Submission

Vol.13, No.3

PubReader Citation

Analysis of Amineptine and its Metabolites in Human Urine by Gas Chromatography/Mass Spectrometry

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985

2000, v.13 no.3, pp.385-393

Lee, Jeong Ae
Kim, Younglim
Lho, Dong-Seok

Lee, J. A., Kim, Y., & Lho, D. (2000). Analysis of Amineptine and its Metabolites in Human Urine by Gas Chromatography/Mass Spectrometry. , 13(3), 385-393.

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Abstract

A gas chromatography-mass spectrometric (GC/MS) procedure for the determination of amineptine (dihydro-10, 11-dibenzo[a, d] cycloheptenyl-5-amino-7-heptanoic acid) and its main metabolites in human urine was described. Amineptine has been known to be extensively metabolized by the ${\beta}$-oxidation of the heptanoic side chain with formation of pentanoic side chain metabolite ($C_5$-metabolite), and lactamizarion by internal dehydration of (${\beta}$-oxidized metabolite (${\delta}$-lactam). The detection of these compounds was based on acid hydrolysis, liquid-liquid extraction and trimethylsilylated derivatization of the carboxylic acid group. For the determination of amineptine and its metabolites in biological fluids, selected ions at the m/ 192, molecular ion and one of the characteristic ions were monitored by GC/MS. On the excretion study of amineptine in human urine, 70-90% of amineptine, ${\delta}$-lactam, and $C_5$-metabolite were found to be excreted within 4 hours and their excretion completed within 20 hours.