Trace Analysis of Racemic Isomers of Flurbiprofen in Human Urine using Column Switching-HPLC

차기원; 최현철; 강신정; 윤미옥; 이수정; 김호정; 박창훈

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P-ISSN 1225-0163
E-ISSN 2288-8985

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Vol.15, No.6

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Trace Analysis of Racemic Isomers of Flurbiprofen in Human Urine using Column Switching-HPLC

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985

2002, v.15 no.6, pp.529-533

& (2002). Trace Analysis of Racemic Isomers of Flurbiprofen in Human Urine using Column Switching-HPLC. , 15(6), 529-533.

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Abstract

The separation and determination method of racemic isomers of flurbiprofen in urine samples have been investigated using column switching- HPLC, which include the sample treatment and concentration column. The optical rotation of two isomers separated were measured to identify d-form and l-form. The calibration curves are liear in the ranges of $0.11-5.4{\mu}g/mL$ for both d-form and l-form. The detection limits were $0.031{\mu}g/mL$ for l-isomes and $0.027{\mu}g/mL$ for d-isomer. The coefficients of variation of intra-day and inter-day precision of this method were about 1.8%. The present method was apllied to determine the concentration of racemic isomers in urine sample from human eating the drug.