- P-ISSN 1225-0163
- E-ISSN 2288-8985
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
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Characterization of biotin-avidin recognition system constructed on the solid substrate
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2005, v.18 no.6, pp.460-468
(2005). Characterization of biotin-avidin recognition system constructed on the solid substrate. , 18(6), 460-468.
Abstract
The biotin-avidin complex, as a model recognition system, has been constructed through N-hydroxysuccinimide(NHS) reaction on a variety of substrates such as a smooth Au film, electrochemically roughened Au electrode and chemically modified mica. Stepwise self-assembled monolayers (SAMs) of biotin-avidin system were characterized by surface-enhanced resonance Raman scattering (SERRS) spectroscopy, atomic force microscopy (AFM) and surface plasmon resonance (SPR). A strong SERRS signal of rhodamine tags labeled in avidin from the SAMs on a roughened gold electrode indicated the successful complex formation of stepwise biotin-avidin recognition system. AFM images showed the circular shaped avidin aggregates (hexamer) with ca.
- keywords
- Biotin, avidin, stepwise self-assembly, SERRS, AFM, SPR
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