- P-ISSN 1225-0163
- E-ISSN 2288-8985
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
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Analysis of dutasteride in human serum by LC-MS/MS
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2012, v.25 no.1, pp.76-82
https://doi.org/10.5806/AST.2012.25.1.076
&
(2012). Analysis of dutasteride in human serum by LC-MS/MS. , 25(1), 76-82, https://doi.org/10.5806/AST.2012.25.1.076
Abstract
The determination and confirmation of dutasteride in human serum was performed by a liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI/MS/MS). Beclomethasone as an internal standard (I.S.) was added to the serum and the mixed sample was pretreated by liquid-liquid extraction (LLE) with methyl tert-butyl ether (MTBE). The mass transitions of dutasteride and I.S. monitored in multiple reaction monitoring (MRM) were m/z 529.6→461.5 and m/z 409.3→391.2, respectively, and the retention times were 6.45 and 5.46min, respectively. The calibration curve was linear in the concentration range of 0.5~30.0 ng/mL (R2= 0.9999) and the limit of quantitation (LOQ) was found to be 0.5 ng/mL. The recovery of dutasteride was shown to be 66~72%. The intra-day assay precision and accuracy were in the range 3.5~5.5% and 85.7~89.9%, respectively, and the interday assay precision and accuracy were in the range 4.2~5.8% and 90.8~95.8%, respectively
- keywords
- Dutasteride, LC-MS/MS, human serum
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