Effect of urea on blood-luminol chemiluminescence reaction

Bae Youngpyo; 배영표; Hong Sungwook; 홍성욱

doi:10.5806/ast.2023.36.6.332

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P-ISSN1225-0163
E-ISSN2288-8985
SCOPUS, ESCI, KCI

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P-ISSN 1225-0163
E-ISSN 2288-8985

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Vol.36, No.6

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Effect of urea on blood-luminol chemiluminescence reaction

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985

2023, v.36 no.6, pp.332-339

https://doi.org/10.5806/ast.2023.36.6.332

Youngpyo Bae (Graduate School of Forensic Science, Soonchunhyang University)
Sungwook Hong (Graduate School of Forensic Science, Soonchunhyang University)

Bae, Y., & Hong, S. (2023). Effect of urea on blood-luminol chemiluminescence reaction. , 36(6), 332-339, https://doi.org/10.5806/ast.2023.36.6.332

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Abstract

This study aimed to verify the hypothesis that urea denatures hemoglobin in the blood, thereby exposing active sites of enzymes and enhancing the chemiluminescence of the blood-luminol reaction. When blood was pretreated with urea, higher concentrations of pretreatment urea or longer pretreatment times resulted in enhanced chemiluminescence in the blood-luminol reaction, supporting the above hypothesis. However, the chemiluminescence was enhanced when blood was treated with luminol mixed with an 8 M urea solution, although the fact that the time for urea to denature hemoglobin was shorter compared to when blood was pretreated with urea and followed by luminol. In addition, the chemiluminescence was enhanced when a transition metal without hemoglobin was reacted with urea-containing luminol. Based on these results, it is anticipated that urea not only denatures hemoglobin but also plays a role in the luminol-hydrogen peroxide reaction.