Journal of the Korean Association of Oral and Maxillofacial Surgeons
- P-ISSN2234-7550
- E-ISSN2234-5930
- SCOPUS, KCI, ESCI
ISSN : 2234-7550
Evaluation of titanium-prepared platelet-rich fibrin incorporated with amoxiclav, metronidazole, and neem gel on bone regeneration in Wistar rats: a pilot study
Damera Trinath Kishore (Department of Periodontology, GITAM Dental College and Hospital, Visakhapatnam, India)
Rampalli Viswa Chandra (Department of Periodontology and Implantology, SVS Institute of Dental Sciences, Mahabubnagar, India)
Boddeda Anusha (Department of Periodontology, GITAM Dental College and Hospital, Visakhapatnam, India)
Gidijala Naveen Vital Kumar (Department of Periodontology, GITAM Dental College and Hospital, Visakhapatnam, India)
Boyapati Ramanarayana (Department of Periodontology, SIBAR Institute of Dental Sciences, Guntur, India)
Gurumoorthy Kaarthikeyan (Department of Periodontology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Chennai, India)
Abstract
Objectives: Titanium platelet-rich fibrin (T-PRF) usage as sustained drug delivery system (SDDS) has been recently started. Hence present study aimed to evaluate hard tissue regenerative efficacy of T-PRF clots incorporated with amoxiclav (AMX)/metronidazole (MET)/neem (NE) gels individually in osseous defects created in Wistar rats. Materials and Methods: In this in vivo animal model trial, 12 female Wistar rats were procured and subjected to ligature induced periodontitis. Bone defects were created and incorporated with T-PRF plain, T-PRF clots+AMX/MET/NE gels individually. Rats were sacrificed after 2 months and checked under light microscopy and micro (μ)-computed tomography analysis for bone regeneration. Frequency distribution percentages, chi-square test, ANOVA and un-paired t -test were used for conducting the statistical analysis. Results: Inflammatory response was reported in T-PRF+MET Group followed by AMX Group and no inflammatory response was recorded for T-PRF plain with statistical significance (P=0.008), for mineralized and un-mineralized bone formation there was no significant difference (P=0.133). Mean comparisons of bone density there was no significant difference (P=0.545) among all (AMX=551.00±18.68, MET=578.00±18.25, NE=569.00±24.58, T-PRF plain=507.67±118.50). For gene expression significantly (P<0.001) higher alkaline phosphatase and bone morphogenetic protein-2 levels were recorded for T-PRF+NE Group (7.60±3.04, 10.85±1.28) followed by MET (4.65±1.52, 4.64±0.45)>AMX (2.47±0.84, 1.89±0.18)>T-PRF plain (1.00±0.00, 1.00±0.00). Conclusion: Research results showed that T-PRF can be a SDDS and addition of antibiotics or herbal extract didn’t alter the hard tissue healing property of T-PRF and resulted in new bone formation.
- keywords
- Amoxicillin, Disease, Health, Metronidazole, Platelet-rich fibrin
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