Characterization of N-Alkylamides of Spilanthes acmella var calva by Using High Performance Liquid Chromatography Electrospray Ionization Ion Trap Mass Spectrometry
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2025, v.16 no.2, pp.31-41
https://doi.org/10.5478/10.5478/MSL.2025.16.2.31
Sajan L. Shyaula
(Faculty of Science, Nepal Academy of Science and Technology)
Ankita Acharya
(Central Department of Chemistry, Tribhuvan University)
Muhammad Iqbal Choudhary
(H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi)
Syed Ghulam Musharraf
(H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi)
Rukesh Maharjan
(H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi)
Arslan Ali
(H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi)
Shyaula,
S. L., Acharya,
A., Choudhary,
M. I., Musharraf,
S. G., Maharjan,
R., &
Ali,
A.
(2025). . , 16(2), 31-41, https://doi.org/10.5478/10.5478/MSL.2025.16.2.31
Abstract
Spilanthes acmella var calva popularly known as ‘Marauti’ in Nepal, are widely used by different communities as
the food ingredients in their traditional cuisines. It displays a unique tingling sensation mainly due to N-alkylamides when
applied to mucosal surfaces. Spilanthes served as a remedy for alleviating toothache, throat infections and other oral ailments. N-
Alkylamide profilings of Spilanthes extracts were performed using a gradient reversed phase high performance liquid chroma-
tography/ electrospray ionization ion trap mass spectrometry (HPLC/ESI-MS) method. A total of 33 peaks were analyzed and 16
N-alkylamides were identified. Seven compounds (1, 5, 6, 8, 15, 16 and 25) were N-isobutylamides and nine compounds (4, 7,
10, 11, 12, 14, 17, 18 and 20) were 2-phenylethylamides.
- keywords
-
Spilanthes acmella,
N-Alkylamides,
N-Isobutylamides,
2-Phenylethylamide,
Ion trap
- 투고일Received
- 2025-02-20
- 수정일Revised
- 2025-04-29
- 게재확정일Accepted
- 2025-05-15
- 출판일Published
- 2025-06-30