Abstract

Chrozophora brocchiana is widely used in traditional medicine in Niger. In this study, hexanic, DCM, AcOEt, and EtOH-H 2 O (70:30) fractions of Chrozophora brocchiana aerial parts were analyzed via ultra-high-performance liquid chroma- tography with high-resolution mass spectrometry (UHPLC-HRMS)-based metabolomics. We explore chemical space with an advanced Global Natural Product Social (GNPS)-based molecular networking (MN) analysis tool, MolNetEnhancer. As result, three superclasses were identified (phenylpropanoids and polyketides; lipids and lipid-like molecules; organoheterocyclic com- pounds). Flavonoids were the most dominant class, followed by tannins, lipids, and lipid-like molecules, and alkaloids. Additio- nally, some compounds' putative structure and molecular formula were manually determined. This novel mapping of the metabolomic landscape of C.brocchiana provides actionable insights into plant biochemistry and justifies certain medicinal uses.