Toxic Evaluation and Chromatographic Analysis of Cucurbitacin D and F from Sorbaria sorbifolia

Lee, Sang-Myung; 이상명; Lee, Cheal-Gyu; 이철규

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P-ISSN 1225-0163
E-ISSN 2288-8985

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Vol.14, No.2

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Toxic Evaluation and Chromatographic Analysis of Cucurbitacin D and F from Sorbaria sorbifolia

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985

2001, v.14 no.2, pp.191-195

Lee, Sang-Myung
Lee, Cheal-Gyu

Lee, S., & Lee, C. (2001). Toxic Evaluation and Chromatographic Analysis of Cucurbitacin D and F from Sorbaria sorbifolia. , 14(2), 191-195.

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Abstract

Cucurbitacin D and F, the protostane type triterpenoid of S. sorbifolia, were isolated with chromatograpic method and used as the standard substances for quantitative analysis. The compounds were identified with $^1H$-NMR, FAB-MS and UV spectrophotometer. They were separated on YMC-Pack ODS-AQ(303)[$250{\times}4.6mm$ I.D., $S-5{\mu}m$, 120A] column by HPLC. Cucurbitacin F was detected at 10.73mg/kg in cortex of S. sorbifolia, but cucurbitacin D was not. The compounds were shown to exihibit significant cytotoxicity($ED_{50}$<$0.1{\mu}g/mL$) against several tumor cell lines and acute toxicity(cucurbitacin D: 4.7mg/kg/day, cucurbitacin F: 2.5mg/kg/day) against BDF-1 mouse.