- P-ISSN 1225-0163
- E-ISSN 2288-8985
A simple and sensitive determination method of histidine has been investigated using the spectrofluorometry. An emission peak of Eu3+ - TTA- histidine complex occurs at 470 nm in aquous solution with excitation at 235 nm and the fluorescence intensity increased linearly with addition of histidine in the range of 1 x 10-7 - 4 x 10-6 M. The detection limits was 5 x 10-7 M with 3.5% RSD. The present method was applied to determine histidine content in the synthetic sample.