Development of the Discrimination Methods for Grographical Origin of Braken(Preridium aquilinum) by Capillary Electrophoresis

류미라; 김은영; 김정현; 정경숙

오늘 하루 그만보기

P-ISSN1225-0163
E-ISSN2288-8985
SCOPUS, ESCI, KCI

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P-ISSN 1225-0163
E-ISSN 2288-8985

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Vol.17, No.2

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Development of the Discrimination Methods for Grographical Origin of Braken(Preridium aquilinum) by Capillary Electrophoresis

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985

2004, v.17 no.2, pp.192-197

& (2004). Development of the Discrimination Methods for Grographical Origin of Braken(Preridium aquilinum) by Capillary Electrophoresis. , 17(2), 192-197.

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Abstract

The discrimination of bracken (Korean vs. Chinese) has been attempted using capillary electrophoresis (CE). Bracken (Pteridium aquilinum) was extracted with 30% methanol and separated on a uncoated fused-silica ($50{\mu}m{\times}27cm$) capillary. Conditions for optimal analysis include: temperature, $40^{\circ}C$; voltage, 8 kV; and pressure injection time, 5 sec. The optimal separation buffer was 0.3 M borate buffer (pH 8.5) containing 40 mM CHAPS with 30% ethylene glycol. Under the optimal conditions established for CE, the ratio of specific peak area (peak PA-1) to other peak area (peak PA-2) was effective in discrimination of Korean and Chinese bracken. The mean accuracy for discrimination of Korean and Chinese brackens were 80% and 86%, respectively.