Characteristics of <TEX>$G_{418}$</TEX>-sensitive mitochondrial ATPase/ATP synthase from pleurotus florida

Kim, Jae-Woong; 김재웅; Kim, Dong-Hee; 김동희; Lee, Jung-Bock; 이정복; Lee, Sur-Koo; 이서구; Min, Tae-Jin; 민태진

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P-ISSN1225-0163
E-ISSN2288-8985
SCOPUS, ESCI, KCI

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P-ISSN 1225-0163
E-ISSN 2288-8985

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Vol.5, No.4

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Characteristics of $G_{418}$-sensitive mitochondrial ATPase/ATP synthase from pleurotus florida

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985

1992, v.5 no.4, pp.477-484

Kim, Jae-Woong
Kim, Dong-Hee
Lee, Jung-Bock
Lee, Sur-Koo
Min, Tae-Jin

Kim, J., Kim, D., Lee, J., Lee, S., & Min, T. (1992). Characteristics of <TEX>$G_{418}$</TEX>-sensitive mitochondrial ATPase/ATP synthase from pleurotus florida. , 5(4), 477-484.

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Abstract

The mitochondrion was purified at 44% sucrose layer from pleurotus florida by using ultracentrifuge and sucrose density gradient method. Optimum pH and temperature of ATPase and ATP synthase were pH 7.4, $60^{\circ}C$ and pH 7.5, $57^{\circ}C$ respectively, also their Km values were determined as 11.6mM and 8.4mM. ATPase was activated at 5~6mM ATP substrate concentration, then ATP synthase was 5~10mM range ADP. ATPase/ATP synthase were $Mg^{2+}$-dependent enzyme, partially inhibited by their substrate, and then showed an none competitive inhibition pattern by $G_{418}$. Amino acid composition of ATPase/ATP synthase was as follows, hydrophobic amino acid residue was 50.5%, small residue, 56.1%, hydrogen bonding residue, 43.7% and helix breaking residue, 55.2%. Phosphatidyl choline, phosphatidyl ethanolamine and phosphatidyl glycerol were contained but not phosphatidyl inositol and phosphatidyl serine. Palmitate(51.31%), stearate(18.32%) and unsaturated fatty acids($C_{18:1}$, $C_{18:2}$ and $C_{16:1}$) were predominated.