Assay of <TEX>$\beta$</TEX>-Galactosidase Using High Performance Liquid Chromatography

Shin, Myung Gon; 신명곤; Chang, Pahn Shick; 장판식; Min, Bong Kee; 민봉기; Kim, Sun Chang; 김선창

오늘 하루 그만보기

P-ISSN1225-0163
E-ISSN2288-8985
SCOPUS, ESCI, KCI

Home

Browse Articles

Article Detail

Home > Article Detail

P-ISSN 1225-0163
E-ISSN 2288-8985

e-Submission

Vol.5, No.4

PubReader Citation

Assay of $\beta$-Galactosidase Using High Performance Liquid Chromatography

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985

1992, v.5 no.4, pp.465-469

Shin, Myung Gon
Chang, Pahn Shick
Min, Bong Kee
Kim, Sun Chang

Shin, M. G., Chang, P. S., Min, B. K., & Kim, S. C. (1992). Assay of <TEX>$\beta$</TEX>-Galactosidase Using High Performance Liquid Chromatography. , 5(4), 465-469.

copy

Abstract

An analytical procedure is presented for the quantitative determination of lactose, glucose, and galactose in the hydrolyzate of lactose by ${\beta}$-galactosidase with high-performance liquid chromatography. An Aminex HPX-87C column at $85^{\circ}C$ and refractive index detector were used to resolve lactose, glucose, and galactose in only 12 minutes with distilled and deionized water as a mobile phase. The validity of high-performance liquid chromatography as a method for the assay of ${\beta}$-galactosidase was supported by recovery experiments and comparision of results with those by ONPG method, a spectrophotometric assay. The procedure was appropriate for determination of sugars in the enzyme reaction mixture and could by applied to analysis of ${\beta}$-galactosidase activity.