An Analytical Method of Thromboxane <TEX>$B_2$</TEX> by Fast Atom Bombardment Mass Spectrometry

Jang, Suk-Yoon; 장석윤; Kim, Jung-Hoon; 김정훈; Lee, Yong-Moon; 이용문; Jang, Seung-Ki; 장성기; Moon, Dong-Cheul; 문동철

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P-ISSN1225-0163
E-ISSN2288-8985
SCOPUS, ESCI, KCI

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P-ISSN 1225-0163
E-ISSN 2288-8985

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Vol.6, No.4

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An Analytical Method of Thromboxane $B_2$ by Fast Atom Bombardment Mass Spectrometry

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985

1993, v.6 no.4, pp.349-357

Jang, Suk-Yoon
Kim, Jung-Hoon
Lee, Yong-Moon
Jang, Seung-Ki
Moon, Dong-Cheul

Jang, S., Kim, J., Lee, Y., Jang, S., & Moon, D. (1993). An Analytical Method of Thromboxane <TEX>$B_2$</TEX> by Fast Atom Bombardment Mass Spectrometry. , 6(4), 349-357.

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Abstract

Analytical methods of thromboxane $B_2(TXB_2)$ using various techniques of Fast Atom Bombardment mass spectrometry (FAB MS) were studied, static FAB condition was investigated to obtain linear response curve using docosanoic acid as a internal standard. For maximum sensitivity, a continuos-flow(CF-) FAB MS by selected-ion monitoring(SIM) with devised sample introduction system, has been developed to quantiate thromboxane $B_2$ in biological sample. Instrumental parameters affecting sensitivity, reproducibility has been studied. The method has been optimized with respect to the eluent, 0.75% glycerol(in EtOH v/v) and flow rate of $3.7{\mu}l/min.$ Under the condition, detection limits were below 10pg in SIM mode and a good linear relationship between dose and response was achieved.