Determination of Sodium Chondroitin Sulfate by Enzymatic Digestion and HPLC

Kang, Seong-Ho; 강성호; Shin, Hoon; 신훈; Chang, Sun-Ki; 장순기; Yoon, Hyung-Jung; 윤형중

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P-ISSN1225-0163
E-ISSN2288-8985
SCOPUS, ESCI, KCI

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P-ISSN 1225-0163
E-ISSN 2288-8985

e-Submission

Vol.7, No.2

PubReader Citation

Determination of Sodium Chondroitin Sulfate by Enzymatic Digestion and HPLC

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985

1994, v.7 no.2, pp.245-251

Kang, Seong-Ho
Shin, Hoon
Chang, Sun-Ki
Yoon, Hyung-Jung

Kang, S., Shin, H., Chang, S., & Yoon, H. (1994). Determination of Sodium Chondroitin Sulfate by Enzymatic Digestion and HPLC. , 7(2), 245-251.

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Abstract

In order to determine sodium chondroitin sulfate in the mixture, chondroitinase ABC was used for enzymatic reaction. The procedure was rapid, simple, quantitative and the HPLC analysis of ${\Delta}Di-6S$(2-actamido-2-deoxy-3-0-(${\beta}$-D-gluco-4-enepyranosyluronic acid)-6-0-sulfo-D-galactose) in the sodium chondroitin sulfate was obtained. The absorbance was measured at 230nm and detection limit was $1{\mu}g/ml$. When we applied this method to the drugs(capsule, opthalmic solution), it gave the mean contents of $100.01{\pm}1.58%$ and $99.89{\pm}1.80%$ respectively.